caa a22 4500 606208585 CHVBK 20210128101023.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00425-014-2217-9 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2217-9 Expression divergence of cellulose synthase ( CesA ) genes after a recent whole genome duplication event in Populus [Elektronische Daten] [Naoki Takata, Toru Taniguchi] Main conclusion : Secondary cell wall-associated CesA genes in Populus have undergone a functional differentiation in expression pattern that may be attributable to evolutionary alteration of regulatory modules. Gene duplication is an important mechanism for functional divergence of genes. Secondary cell wall-associated cellulose synthase genes (CesA4, CesA7 and CesA8) are duplicated in Populus plants due to a recent whole genome duplication event. Here, we demonstrate that duplicate CesA genes show tissue-dependent expression divergence in Populus plants. Real-time PCR analysis of Populus CesA genes suggested that Pt×tCesA8-B was more highly expressed than Pt×tCesA8-A in phloem and secondary xylem tissue of mature stem. Histochemical and histological analyses of transformants expressing a GFP-GUS fusion gene driven by Populus CesA promoters revealed that the duplicate CesA genes showed different expression patterns in phloem fibers, secondary xylem, root cap and leaf trichomes. We predicted putative cis-regulatory motifs that regulate expression of secondary cell wall-associated CesA genes, and identified 19 motifs that are highly conserved in the CesA gene family of eudicotyledonous plants. Furthermore, a transient transactivation assay identified candidate transcription factors that affect levels and patterns of expression of Populus CesA genes. The present study reveals that secondary cell wall-associated CesA genes in Populus have undergone a functional differentiation in expression pattern that may be attributable to evolutionary alteration of regulatory modules. Springer-Verlag Berlin Heidelberg, 2014 Cellulose synthase nationallicence Functional divergence nationallicence Gene duplication nationallicence Secondary cell wall nationallicence Whole genome duplication nationallicence CaMV35S : Cauliflower mosaic virus 35S nationallicence CesA : Cellulose synthase nationallicence CSC : Cellulose synthase complex nationallicence GFP : Green fluorescent protein nationallicence EmGFP : Emerald green fluorescent protein nationallicence GUS : β-Glucuronidase nationallicence JTT : Jones-Taylor-Thornton nationallicence LBD : LOB domain-containing protein nationallicence LUC : Luciferase nationallicence M46RE : MYB46-responsive cis-element nationallicence MBSIIG : MYB binding site IIG nationallicence NAC : NAM, ATAF1/2 and CUC2 nationallicence NJ : Neighbor-joining nationallicence RLUC : Renilla reniformis luciferase nationallicence UBQ : Ubiquitin nationallicence WGD : Whole genome duplication nationallicence ZF : Zinc-finger protein nationallicence Takata Naoki Forest Bio-Research Center, Forestry and Forest Products Research Institute, 319-1301, Hitachi, Ibaraki, Japan aut Taniguchi Toru Forest Bio-Research Center, Forestry and Forest Products Research Institute, 319-1301, Hitachi, Ibaraki, Japan aut Planta Springer Berlin Heidelberg 241/1(2015-01-01), 29-42 0032-0935 241:1<29 2015 241 425 https://doi.org/10.1007/s00425-014-2217-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2217-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takata Naoki Forest Bio-Research Center, Forestry and Forest Products Research Institute, 319-1301, Hitachi, Ibaraki, Japan aut NATIONALLICENCE 700 1- Taniguchi Toru Forest Bio-Research Center, Forestry and Forest Products Research Institute, 319-1301, Hitachi, Ibaraki, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/1(2015-01-01), 29-42 0032-0935 241:1<29 2015 241 425