caa a22 4500 606208658 CHVBK 20210128101023.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00425-014-2160-9 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2160-9 An anther development F-box (ADF) protein regulated by tapetum degeneration retardation (TDR) controls rice anther development [Elektronische Daten] [Li Li, Yixing Li, Shufeng Song, Huafeng Deng, Na Li, Xiqin Fu, Guanghui Chen, Longping Yuan] Main conclusion : In this study, we reported that a F-box protein, OsADF, as one of the direct targets of TDR , plays a critical role in rice tapetum cell development and pollen formation. Abstract: The tapetum, the innermost sporophytic tissue of anther, plays an important supportive role in male reproduction in flowering plants. After meiosis, tapetal cells undergo programmed cell death (PCD) and provide nutrients for pollen development. Previously we showed that tapetum degeneration retardation (TDR), a basic helix-loop-helix transcription factor, can trigger tapetal PCD and control pollen wall development during anther development. However, the comprehensive regulatory network of TDR remains to be investigated. In this study, we cloned and characterized a panicle-specific expression F-box protein, anther development F-box (OsADF). By qRT-PCR and RNA in situ hybridization, we further confirmed that OsADF expressed specially in tapetal cells from stage 9 to stage 12 during anther development. In consistent with this specific expression pattern, the RNAi transgenic lines of OsADF exhibited abnormal tapetal degeneration and aborted microspores development, which eventually grew pollens with reduced fertility. Furthermore, we demonstrated that the TDR, a key regulator in controlling rice anther development, could regulate directly the expression of OsADF by binding to E-box motifs of its promoter. Therefore, this work highlighted the possible regulatory role of TDR, which regulates tapetal cell development and pollen formation via triggering the possible ADF-mediated proteolysis pathway. Springer-Verlag Berlin Heidelberg, 2014 Rice nationallicence Male sterility nationallicence Tapetum nationallicence TDR nationallicence PCD nationallicence OsADF nationallicence Li Li State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut Li Yixing Hunan Agricultural University, 410128, Changsha, China aut Song Shufeng State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut Deng Huafeng State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut Li Na State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut Fu Xiqin State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut Chen Guanghui Collaborative Innovation Center for Modern Production of Multiple Cropping System in Southern Paddy Area, 410128, Changsha, China aut Yuan Longping State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut Planta Springer Berlin Heidelberg 241/1(2015-01-01), 157-166 0032-0935 241:1<157 2015 241 425 https://doi.org/10.1007/s00425-014-2160-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2160-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Li State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut NATIONALLICENCE 700 1- Li Yixing Hunan Agricultural University, 410128, Changsha, China aut NATIONALLICENCE 700 1- Song Shufeng State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut NATIONALLICENCE 700 1- Deng Huafeng State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut NATIONALLICENCE 700 1- Li Na State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut NATIONALLICENCE 700 1- Fu Xiqin State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut NATIONALLICENCE 700 1- Chen Guanghui Collaborative Innovation Center for Modern Production of Multiple Cropping System in Southern Paddy Area, 410128, Changsha, China aut NATIONALLICENCE 700 1- Yuan Longping State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Centre, 410125, Changsha, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/1(2015-01-01), 157-166 0032-0935 241:1<157 2015 241 425