caa a22 4500 606208666 CHVBK 20210128101023.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00425-014-2161-8 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2161-8 Stress signaling in response to polycyclic aromatic hydrocarbon exposure in Arabidopsis thaliana involves a nucleoside diphosphate kinase, NDPK-3 [Elektronische Daten] [Hong Liu, David Weisman, Ling Tang, Long Tan, Wen-ke Zhang, Zong-hua Wang, Yan-he Huang, Wen-xiong Lin, Xuan-ming Liu, Adán Colón-Carmona] Main conclusion : The study is the first to reveal the proteomic response in plants to a single PAH stress, and indicates that NDPK3 is a positive regulator in the Arabidopsis response to phenanthrene stress. Abstract: Polycyclic aromatic hydrocarbons (PAHs) are highly carcinogenic pollutants that are byproducts of carbon-based fuel combustion, and tend to persist in the environment for long periods of time. PAHs elicit complex, damaging responses in plants, and prior research at the physiological, biochemical, and transcriptional levels has indicated that reactive oxygen species (ROS) and oxidative stress play major roles in the PAH response. However, the proteomic response has remained largely unexplored. This study hypothesized that the proteomic response in Arabidopsis thaliana to phenanthrene, a model PAH, would include a strong oxidative stress signature, and would provide leads to potential signaling molecules involved. To explore that proteomic signature, we performed 2D-PAGE experiments and identified 30 proteins levels that were significantly altered including catalases (CAT), ascorbate peroxidase (APX), peroxiredoxins (POD), glutathione-S-transferase, and glutathione reductase. Also upregulated was nucleoside diphosphate kinase 3 (NDPK-3), a protein known to have metabolic and stress signaling functions. To address whether NDPK-3 functions upstream of the oxidative stress response, we measured levels of stress-responsive enzymes in NDPK-3 overexpressor, loss-of-function knockout, and wild-type plant lines. In the NDPK-3 overexpressor, the enzyme activities of APX, CAT, POD, as well as superoxide dismutase were all increased compared to wild type; in the NDPK-3 knockout line, these enzymes had reduced activity. This pattern occurred in untreated as well as phenanthrene-treated plants. These data support a model in which NDPK-3 is a positive regulator of the Arabidopsis stress response to PAHs. Springer-Verlag Berlin Heidelberg, 2014 Abiotic stress nationallicence Nucleoside diphosphate kinase-3 nationallicence Plant nationallicence Phenanthrene nationallicence Positive regulator nationallicence APX : Ascorbate peroxidase nationallicence CAT : Catalases nationallicence MDA : Malondialdehyde nationallicence NDPK-3 : Nucleoside diphosphate kinase 3 nationallicence PAH : Polycyclic aromatic hydrocarbons nationallicence PCD : Programmed cell death nationallicence POD : Peroxiredoxins nationallicence ROS : Reactive oxygen species nationallicence Liu Hong College of Resources and Environment, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Weisman David Department of Biology, University of Massachusetts, 100 Morrissey Blvd, 02125-3393, Boston, MA, USA aut Tang Ling The School of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Tan Long College of Resources and Environment, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Zhang Wen-ke The School of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Wang Zong-hua The School of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Huang Yan-he College of Resources and Environment, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Lin Wen-xiong The School of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut Liu Xuan-ming Department of Life Science, College of Biology, Hunan University, 410082, Changsha, China aut Colón-Carmona Adán Department of Biology, University of Massachusetts, 100 Morrissey Blvd, 02125-3393, Boston, MA, USA aut Planta Springer Berlin Heidelberg 241/1(2015-01-01), 95-107 0032-0935 241:1<95 2015 241 425 https://doi.org/10.1007/s00425-014-2161-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2161-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Hong College of Resources and Environment, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Weisman David Department of Biology, University of Massachusetts, 100 Morrissey Blvd, 02125-3393, Boston, MA, USA aut NATIONALLICENCE 700 1- Tang Ling The School of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Tan Long College of Resources and Environment, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Zhang Wen-ke The School of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Wang Zong-hua The School of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Huang Yan-he College of Resources and Environment, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Lin Wen-xiong The School of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China aut NATIONALLICENCE 700 1- Liu Xuan-ming Department of Life Science, College of Biology, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Colón-Carmona Adán Department of Biology, University of Massachusetts, 100 Morrissey Blvd, 02125-3393, Boston, MA, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/1(2015-01-01), 95-107 0032-0935 241:1<95 2015 241 425