caa a22 4500 606208674 CHVBK 20210128101024.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00425-014-2170-7 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2170-7 Differential regulation of caffeine metabolism in Coffea arabica (Arabica) and Coffea canephora (Robusta) [Elektronische Daten] [Charlène Perrois, Susan Strickler, Guillaume Mathieu, Maud Lepelley, Lucie Bedon, Stéphane Michaux, Jwanro Husson, Lukas Mueller, Isabelle Privat] Caffeine is a metabolite of great economic importance, especially in coffee, where it influences the sensorial and physiological impacts of the beverage. Caffeine metabolism in the Coffea species begins with the degradation of purine nucleotides through three specific N-methyltransferases: XMT, MXMT and DXMT. A comparative analysis was performed to clarify the molecular reasons behind differences in caffeine accumulation in two Coffea species, namely Coffea arabica and Coffea canephora var. robusta. Three different genes encoding N-methyltransferase were amplified in the doubled haploid Coffea canephora: CcXMT1, CcMXMT1 and CcDXMT. Six genes were amplified in the haploid Coffea arabica: CaXMT1, CaXMT2, CaMXMT1, CaMXMT2, CaDXMT1, and CaDXMT2. A complete phylogenic analysis was performed to identify specific key amino acids defining enzymatic function for each protein identified. Furthermore, a quantitative gene-expression analysis was conducted on leaves and on maturing coffee beans, simultaneously analyzing caffeine content. In the different varieties analyzed, caffeine accumulation is higher in leaves than in the coffee bean maturation period, higher in Robusta than in Arabica. In Robusta, CcXMT1 and CcDXMT gene expressions are predominant and transcriptional activity is higher in leaves than in maturing beans, and is highly correlated to caffeine accumulation. In Arabica, the CaXMT1 expression level is high in leaves and CaDXMT2 as well to a lesser extent, while global transcriptional activity is weak during bean maturation, suggesting that the transcriptional control of caffeine-related genes differs within different organs and between Arabica and Robusta. These findings indicate that caffeine accumulation in Coffea species has been modulated by a combination of differential transcriptional regulation and genome evolution. The Author(s), 2014 Arabica nationallicence Beans nationallicence Caffeine nationallicence Expression nationallicence Leaves nationallicence N -methyltransferase nationallicence Robusta nationallicence Ca : Coffea arabica nationallicence Cc : Coffea canephora var. robusta nationallicence Arabica : Coffea arabica nationallicence Robusta : Coffea canephora var. robusta nationallicence rpl39 : Large ribosomal subunit 39 protein nationallicence UBQ : Ubiquitin protein nationallicence q-RT-PCR : Quantitative real-time polymerase chain reaction nationallicence gDNA : Genomic DNA nationallicence XMT : Xanthosine-N-methyltranferase nationallicence MXMT : 7-methylxanthine-N-methyltransferase or theobromine synthase nationallicence DXMT : 3,7-dimethylxanthine-N-methyltranferase or caffeine synthase nationallicence MTL : Methyltransferase-like protein nationallicence Perrois Charlène Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut Strickler Susan Boyce Thompson Institute for Plant Research, Tower Road, 14853, Ithaca, NY, USA aut Mathieu Guillaume Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut Lepelley Maud Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut Bedon Lucie Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut Michaux Stéphane Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut Husson Jwanro Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut Mueller Lukas Boyce Thompson Institute for Plant Research, Tower Road, 14853, Ithaca, NY, USA aut Privat Isabelle Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut Planta Springer Berlin Heidelberg 241/1(2015-01-01), 179-191 0032-0935 241:1<179 2015 241 425 https://doi.org/10.1007/s00425-014-2170-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2170-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Perrois Charlène Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut NATIONALLICENCE 700 1- Strickler Susan Boyce Thompson Institute for Plant Research, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Mathieu Guillaume Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut NATIONALLICENCE 700 1- Lepelley Maud Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut NATIONALLICENCE 700 1- Bedon Lucie Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut NATIONALLICENCE 700 1- Michaux Stéphane Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut NATIONALLICENCE 700 1- Husson Jwanro Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut NATIONALLICENCE 700 1- Mueller Lukas Boyce Thompson Institute for Plant Research, Tower Road, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Privat Isabelle Nestlé R&D Center, 101 Av. Gustave Eiffel, Notre Dame D'Oé, BP 49716, 37097, Tours, France aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/1(2015-01-01), 179-191 0032-0935 241:1<179 2015 241 425