caa a22 4500 606208690 CHVBK 20210128101024.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00425-014-2175-2 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2175-2 Taxonomically restricted genes of Craterostigma plantagineum are modulated in their expression during dehydration and rehydration [Elektronische Daten] [Valentino Giarola, Stephanie Krey, Anneke Frerichs, Dorothea Bartels] Main conclusion : Taxonomically restricted genes are known to contribute to the evolution of new traits. In Craterostigma plantagineum two of such genes are modulated during dehydration and rehydration and seem to contribute to a successful recovery after desiccation. Abstract: The resurrection plant Craterostigma plantagineum can tolerate extreme water loss. Protective molecules linked to desiccation tolerance were identified in C. plantagineum but underlying mechanisms are far from being completely understood. A transcriptome analysis revealed several genes which could not be annotated and are, therefore, interesting candidates for understanding desiccation tolerance. Genes which occur only in some species are defined as orphan or taxonomically restricted genes (TRGs) and may be important for the evolution of new traits. Several of these TRGs are modulated in expression during dehydration/rehydration in C. plantagineum. Here we report the characterisation of two of these TRGs encoding a cysteine-rich rehydration-responsive protein 1(CpCRP1) and an early dehydration-responsive protein1 (CpEDR1). The involvement of CpCRP1 and CpEDR1 in different phases of the dehydration/rehydration cycle is shown by transcript and protein expression analysis. In silico sequence analyses predicted that both genes are likely to interact with other cellular components and are localised in two different cellular compartments. GFP fusion proteins demonstrated that CpCRP1 is secreted into the apoplasm, whereas CpEDR1 is imported into chloroplasts. Putative homologs of CpCRP1 and CpEDR1 were identified in Lindernia brevidens and Lindernia subracemosa which belong to the same family as C. plantagineum thus suggesting a recent evolution of the genes in this family. According to expression profiles, CpCRP1 may play a role in normal conditions and during rehydration, whereas CpEDR1 may be required for the acquisition of desiccation tolerance and protect photosynthetic structures during dehydration and rehydration. Springer-Verlag Berlin Heidelberg, 2014 Desiccation tolerance nationallicence Resurrection plants nationallicence Cysteine-rich protein nationallicence Orphan genes nationallicence Linderniaceae nationallicence Giarola Valentino Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, 53115, Bonn, Germany aut Krey Stephanie Botanical Institute, Cologne Biocenter, University of Cologne, Zülpicherstraße 47b, 50674, Cologne, Germany aut Frerichs Anneke Institute of Developmental Biology, Cologne Biocenter, University of Cologne, Zülpicherstraße 47b, 50674, Cologne, Germany aut Bartels Dorothea Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, 53115, Bonn, Germany aut Planta Springer Berlin Heidelberg 241/1(2015-01-01), 193-208 0032-0935 241:1<193 2015 241 425 https://doi.org/10.1007/s00425-014-2175-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2175-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Giarola Valentino Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Krey Stephanie Botanical Institute, Cologne Biocenter, University of Cologne, Zülpicherstraße 47b, 50674, Cologne, Germany aut NATIONALLICENCE 700 1- Frerichs Anneke Institute of Developmental Biology, Cologne Biocenter, University of Cologne, Zülpicherstraße 47b, 50674, Cologne, Germany aut NATIONALLICENCE 700 1- Bartels Dorothea Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, 53115, Bonn, Germany aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/1(2015-01-01), 193-208 0032-0935 241:1<193 2015 241 425