caa a22 4500 606208712 CHVBK 20210128101024.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00425-014-2163-6 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2163-6 Promotion of crystalline cellulose degradation by expansins from Oryza sativa [Elektronische Daten] [Yasutaka Seki, Yukiko Kikuchi, Ryo Yoshimoto, Kenichi Aburai, Yoshihiro Kanai, Tatsushi Ruike, Kazuki Iwabata, Ryo Goitsuka, Fumio Sugawara, Masahiko Abe, Kengo Sakaguchi] Main conclusion : Enzymatic activities of Oryza sativa expansins, which were heterologously overexpressed in Escherichia coli , were analyzed. Results suggested that expansins promote degradation of cellulose by cellulase in a synergistic manner. Abstract: Sustainable production of future biofuels is dependent on efficient saccharification of lignocelluloses. Expansins have received a lot of attention as proteins promoting biological degradation of cellulose using cellulase. The expansins are a class of plant cell wall proteins that induce cell wall loosening without hydrolysis. In this study, the expansins from Oryza sativa were classified using phylogenetic analysis and five proteins were selected for functional evaluation. At low cellulose loading, the cellulase in expansin mixtures was up to 2.4 times more active than in mixtures containing only cellulase, but at high cellulose loading the activity of cellulase in expansin mixtures and cellulase only mixtures did not differ. Furthermore, expansin activity was greater in cellulase mixtures compared with cellulase-deficient mixtures. Therefore, the expansins showed significant synergistic activity with cellulase. Expansin may play an important role in efficient saccharification of cellulose. Springer-Verlag Berlin Heidelberg, 2014 Cellulase nationallicence HPLC nationallicence X-Ray diffraction nationallicence Pre-reacted experiment nationallicence Addition order experiment nationallicence Synergistic activity nationallicence Seki Yasutaka Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut Kikuchi Yukiko Department of Industrial Chemistry, Tokyo University of Science, Noda, Chiba, Japan aut Yoshimoto Ryo Department of Industrial Chemistry, Tokyo University of Science, Noda, Chiba, Japan aut Aburai Kenichi Department of Industrial Chemistry, Tokyo University of Science, Noda, Chiba, Japan aut Kanai Yoshihiro Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut Ruike Tatsushi Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut Iwabata Kazuki Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut Goitsuka Ryo Department of Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba, Japan aut Sugawara Fumio Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut Abe Masahiko Department of Industrial Chemistry, Tokyo University of Science, Noda, Chiba, Japan aut Sakaguchi Kengo Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut Planta Springer Berlin Heidelberg 241/1(2015-01-01), 83-93 0032-0935 241:1<83 2015 241 425 https://doi.org/10.1007/s00425-014-2163-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2163-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Seki Yasutaka Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut NATIONALLICENCE 700 1- Kikuchi Yukiko Department of Industrial Chemistry, Tokyo University of Science, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Yoshimoto Ryo Department of Industrial Chemistry, Tokyo University of Science, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Aburai Kenichi Department of Industrial Chemistry, Tokyo University of Science, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Kanai Yoshihiro Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut NATIONALLICENCE 700 1- Ruike Tatsushi Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut NATIONALLICENCE 700 1- Iwabata Kazuki Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut NATIONALLICENCE 700 1- Goitsuka Ryo Department of Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Sugawara Fumio Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut NATIONALLICENCE 700 1- Abe Masahiko Department of Industrial Chemistry, Tokyo University of Science, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Sakaguchi Kengo Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, 278-8510, Chiba-Ken, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/1(2015-01-01), 83-93 0032-0935 241:1<83 2015 241 425