caa a22 4500 606208747 CHVBK 20210128101024.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00425-014-2164-5 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2164-5 Spatial and temporal activity of the foxtail millet ( Setaria italica ) seed-specific promoter pF128 [Elektronische Daten] [Yanlin Pan, Xin Ma, Hanwen Liang, Qian Zhao, Dengyun Zhu, Jingjuan Yu] Main conclusion : pF128 drives GUS specifically expressed in transgenic seeds of foxtail millet and Zea mays with higher activity than the constitutive CaMV35S promoter and the maize seed-specific 19Z promoter. Abstract: Foxtail millet (Setaria italica), a member of the Poaceae family, is an important food and fodder crop in arid regions. Foxtail millet is an excellent C4 crop model owing to its small genome (~490Mb), self-pollination and availability of a complete genome sequence. F128 was isolated from a cDNA library of foxtail millet immature seeds. Real-time PCR analysis revealed that F128 mRNA was specifically expressed in immature and mature seeds. The highest F128 mRNA level was observed 5days after pollination and gradually decreased as the seed matured. Sequence analysis suggested that the protein encoded by F128 is likely a protease inhibitor/seed storage protein/lipid-transfer protein. The 1,053bp 5′ flanking sequence of F128 (pF128) was isolated and fused to the GUS reporter gene. The corresponding vector was then transformed into Arabidopsis thaliana, foxtail millet and Zea mays. GUS analysis revealed that pF128 drove GUS expression efficiently and specifically in the seeds of transgenic Arabidopsis, foxtail millet and Zea mays. GUS activity was also detected in Arabidopsis cotyledons. Activity of pF128 was higher than that observed for the constitutive CaMV35S promoter and the maize seed-specific 19 Zein (19Z) promoter. These results indicate that pF128 is a seed-specific promoter. Its application is expected to be of considerable value in plant genetic engineering. Springer-Verlag Berlin Heidelberg, 2014 Foxtail millet nationallicence Lipid-transfer proteins nationallicence Promoter nationallicence Embryo nationallicence Endosperm transfer cell nationallicence Pan Yanlin State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut Ma Xin State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut Liang Hanwen State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut Zhao Qian State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut Zhu Dengyun State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut Yu Jingjuan State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut Planta Springer Berlin Heidelberg 241/1(2015-01-01), 57-67 0032-0935 241:1<57 2015 241 425 https://doi.org/10.1007/s00425-014-2164-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2164-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pan Yanlin State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Ma Xin State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Liang Hanwen State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Zhao Qian State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Zhu Dengyun State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Yu Jingjuan State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/1(2015-01-01), 57-67 0032-0935 241:1<57 2015 241 425