caa a22 4500 606208755 CHVBK 20210128101024.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00425-014-2181-4 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2181-4 Relative deposition of xylan and 8-5′-linked lignin structure in Chamaecyparis obtusa , as revealed by double immunolabeling by using monoclonal antibodies [Elektronische Daten] [Shingo Kiyoto, Arata Yoshinaga, Keiji Takabe] Main conclusion : Immunolabeling by using monoclonal antibodies showed that xylan deposition precedes the formation of 8-5′-linked structure of lignin in normal and compression woods of Chamaecyparis obtusa. Abstract: Xylan deposition and formation of 8-5′-linked lignin structure in differentiating xylems from normal and compression woods in Chamaecyparis obtusa were examined by immunoelectron microscopy using monoclonal antibodies (LM10 or LM11) to detect xylan localization. The 8-5′-linked lignin structure was immunolocalized using KM1 antibody. Xylan and 8-5′-linked lignin double immunolabeling was performed using secondary antibodies labeled with colloidal gold particles of different diameters. In normal wood, KM1 labeling occurred in the compound middle lamella (CML) and S1 layer during S1 layer formation and increased as S2 and S3 layers formed, with labeling occurring at the outer part of the previous layer. In compression wood, mild KM1 labeling occurred in the CML and outer part of the S1 layer at the later S1 layer formation stage, with increased labeling as the S2 layer formed. Minor labeling occurred in the outer part of the S2 layer during helical cavity formation. Comparison between KM1 labeling and KMnO4 staining suggested that lignin other than 8-5′-linked structure was formed during early lignification, and the proportion of 8-5′-linked lignin structure increased at later stages of lignification in both normal and compression woods. LM10 and LM11 labeling occurred slightly earlier than KM1 labeling, suggesting that xylan deposition preceded the formation of 8-5′-linked lignin in normal and compression woods. Less labeling by KM1, LM10, and LM11 occurred in the outer part of the S2 layer in compression wood, which has abundant lignin. Thus, lignin in these parts is composed of lignin substructures other than the 8-5′ linkage. Springer-Verlag Berlin Heidelberg, 2014 Chamaecyparis nationallicence Lignification nationallicence 8-5′ Linkage nationallicence Monoclonal antibody nationallicence Wood cell wall nationallicence Xylan deposition nationallicence BSA : Bovine serum albumin nationallicence CML : Compound middle lamella nationallicence p AHA : p-aminohippuric acid nationallicence TBS : Tris buffered saline nationallicence Kiyoto Shingo Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut Yoshinaga Arata Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut Takabe Keiji Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut Planta Springer Berlin Heidelberg 241/1(2015-01-01), 243-256 0032-0935 241:1<243 2015 241 425 https://doi.org/10.1007/s00425-014-2181-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2181-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kiyoto Shingo Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Yoshinaga Arata Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Takabe Keiji Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/1(2015-01-01), 243-256 0032-0935 241:1<243 2015 241 425