caa a22 4500 606208798 CHVBK 20210128101024.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00425-015-2269-5 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2269-5 Transgenic expression of an unedited mitochondrial orfB gene product from wild abortive (WA) cytoplasm of rice ( Oryza sativa L.) generates male sterility in fertile rice lines [Elektronische Daten] [Anirban Chakraborty, Joy Mitra, Jagannath Bhattacharyya, Subrata Pradhan, Narattam Sikdar, Srirupa Das, Saikat Chakraborty, Sachin Kumar, Suman Lakhanpaul, Soumitra Sen] Main conclusion : Over-expression of the unedited mitochondrial orfB gene product generates male sterility in fertile indica rice lines in a dose-dependent manner. Cytoplasmic male sterility (CMS) and nuclear-controlled fertility restoration are widespread developmental features in plant reproductive systems. In self-pollinated crop plants, these processes often provide useful tools to exploit hybrid vigour. The wild abortive CMS has been employed in the majority of the "three-line” hybrid rice production since 1970s. In the present study, we provide experimental evidence for a positive functional relationship between the 1.1-kb unedited orfB gene transcript, and its translated product in the mitochondria with male sterility. The generation of the 1.1-kb unedited orfB gene transcripts increased during flowering, resulting in low ATP synthase activity in sterile plants. Following insertion of the unedited orfB gene into the genome of male-fertile plants, the plants became male sterile in a dose-dependent manner with concomitant reduction of ATPase activity of F1F0-ATP synthase (complex V). Fertility of the transgenic lines and normal activity of ATP synthase were restored by down-regulation of the unedited orfB gene expression through RNAi-mediated silencing. The genetic elements deciphered in this study could further be tested for their use in hybrid rice development. Springer-Verlag Berlin Heidelberg, 2015 F1F0-ATP synthase nationallicence Hybrid seed production nationallicence Mitochondrial orfB ( mt - orfB ) gene nationallicence Mitochondrial RNA editing nationallicence Transgenic male-sterile plant nationallicence Wild abortive cytoplasmic male sterility (WA-CMS) nationallicence CDS : Coding DNA Sequence nationallicence dsRNA : Double-stranded RNA nationallicence mt - orfB : Mitochondrial orfB nationallicence ORF : Open reading frame nationallicence qRT-PCR : Quantitative reverse transcription PCR nationallicence RNAi : RNA interference nationallicence siRNA : Small interfering RNA nationallicence WA-CMS : Wild-abortive cytoplasmic male sterility nationallicence Chakraborty Anirban Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Mitra Joy Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Bhattacharyya Jagannath Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Pradhan Subrata Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Sikdar Narattam Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Das Srirupa Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Chakraborty Saikat Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Kumar Sachin Department of Botany, University of Delhi, 110007, Delhi, India aut Lakhanpaul Suman Department of Botany, University of Delhi, 110007, Delhi, India aut Sen Soumitra Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1463-1479 0032-0935 241:6<1463 2015 241 425 https://doi.org/10.1007/s00425-015-2269-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2269-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chakraborty Anirban Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 700 1- Mitra Joy Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 700 1- Bhattacharyya Jagannath Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 700 1- Pradhan Subrata Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 700 1- Sikdar Narattam Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 700 1- Das Srirupa Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 700 1- Chakraborty Saikat Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 700 1- Kumar Sachin Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Lakhanpaul Suman Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Sen Soumitra Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1463-1479 0032-0935 241:6<1463 2015 241 425