caa a22 4500 606208801 CHVBK 20210128101024.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00425-015-2263-y doi (NATIONALLICENCE)springer-10.1007/s00425-015-2263-y Proteome analysis of pear reveals key genes associated with fruit development and quality [Elektronische Daten] [Jia Li, Xiao Huang, Lie Li, Dan Zheng, Cheng Xue, Shao Zhang, Jun Wu] Main conclusion : Comparative and association analyses of the proteome and transcriptome for pear fruit development were conducted for the first time in this study. Pear fruit development involves complex physiological and biochemical processes, but there is still little knowledge available at proteomic and transcriptomic levels, which would be helpful for understanding the molecular mechanisms of fruit development and quality in pear. In our study, three important stages, including early development (S4-22), middle development (S6-27), and near ripening (S8-30), were investigated in ‘Dangshansuli' by isobaric tags for relative and absolute quantitation (iTRAQ) labeling technology, identifying a total of 1,810 proteins during pearfruit development. The association analysis of proteins and transcript expression revealed 1,724, 1,722, and 1,718 associated proteins identified in stages S4-22, S6-27, and S8-30, respectively. A total of 237, 318, and 425 unique proteins were identified as differentially expressed during S4-22 vs S6-27, S6-27 vs S8-30, S4-22 vs S8-30, respectively, and the corresponding correlation coefficients of the overall differentially expressed proteins and transcripts data were 0.6336, 0.4113, and 0.7049. The phenylpropanoid biosynthesis pathway, which is related to lignin formation of pear fruit, was identified as a significantly enriched pathway during early stages of fruit development. Finally, a total of 35 important differentially expressed proteins related to fruit quality were identified, including three proteins related to sugar formation, seven proteins related to aroma synthesis, and sixteen proteins related to the formation of lignin. In addition, qRT-PCR verification provided further evidence to support differentially expressed gene selection. This study is the first to reveal protein and associated mRNA variations in pear during fruit development and quality conformation, and identify key genes and proteins helpful for future functional genomics studies, and provides gene resources for improvement of pear quality. Springer-Verlag Berlin Heidelberg, 2015 Pear nationallicence Fruit development nationallicence Proteomic nationallicence Transcriptomic nationallicence Differential expression nationallicence DAFB : Day after full blooming nationallicence iTRAQ : Isobaric tags for relative and absolute quantitation nationallicence SCX : Strong cation exchange nationallicence HPLC : High pressure liquid chromatography nationallicence AGC : Automatic gain control nationallicence HCD : High-energy collision dissociation nationallicence KEGG : Kyoto encyclopedia of genes and genomes nationallicence GO : Gene ontology nationallicence COG : Cluster of orthologous groups of proteins nationallicence PAL : Phenylalanineammonia-lyase nationallicence F5H : Ferulate-5-hydroxylase nationallicence 4CL : 4-coumarate-CoA ligase nationallicence CCR : Cinnamoyl-CoA reductase nationallicence CAD : Cinnamyl-alcoholdehydrogenase nationallicence POD : Peroxidase nationallicence HCT : Hydroxycinnamoyl transferase nationallicence MFS : Major facilitator super family transporter nationallicence Li Jia State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut Huang Xiao State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut Li Lie State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut Zheng Dan Roy J. Carver Biotechnology Center, University of Illinois Urbana-Champaign, Urbana, IL, USA aut Xue Cheng State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut Zhang Shao State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut Wu Jun State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1363-1379 0032-0935 241:6<1363 2015 241 425 https://doi.org/10.1007/s00425-015-2263-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2263-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Jia State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Huang Xiao State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Li Lie State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Zheng Dan Roy J. Carver Biotechnology Center, University of Illinois Urbana-Champaign, Urbana, IL, USA aut NATIONALLICENCE 700 1- Xue Cheng State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Zhang Shao State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 700 1- Wu Jun State Key Laboratory of Crop Genetics and Germplasm Enhancement, Centre of Pear Engineering Technology Research, Nanjing Agricultural University, 210095, Nanjing, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1363-1379 0032-0935 241:6<1363 2015 241 425