caa a22 4500 60620881X CHVBK 20210128101024.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00425-015-2266-8 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2266-8 Osmotic stress represses strigolactone biosynthesis in Lotus japonicus roots: exploring the interaction between strigolactones and ABA under abiotic stress [Elektronische Daten] [Junwei Liu, Hanzi He, Marco Vitali, Ivan Visentin, Tatsiana Charnikhova, Imran Haider, Andrea Schubert, Carolien Ruyter-Spira, Harro Bouwmeester, Claudio Lovisolo, Francesca Cardinale] Main conclusion : Strigolactone changes and cross talk with ABA unveil a picture of root-specific hormonal dynamics under stress. Abstract: Strigolactones (SLs) are carotenoid-derived hormones influencing diverse aspects of development and communication with (micro)organisms, and proposed as mediators of environmental stimuli in resource allocation processes; to contribute to adaptive adjustments, therefore, their pathway must be responsive to environmental cues. To investigate the relationship between SLs and abiotic stress in Lotus japonicus, we compared wild-type and SL-depleted plants, and studied SL metabolism in roots stressed osmotically and/or phosphate starved. SL-depleted plants showed increased stomatal conductance, both under normal and stress conditions, and impaired resistance to drought associated with slower stomatal closure in response to abscisic acid (ABA). This confirms that SLs contribute to drought resistance in species other than Arabidopsis. However, we also observed that osmotic stress rapidly and strongly decreased SL concentration in tissues and exudates of wild-type Lotus roots, by acting on the transcription of biosynthetic and transporter-encoding genes and independently of phosphate abundance. Pre-treatment with exogenous SLs inhibited the osmotic stress-induced ABA increase in wild-type roots and down-regulated the transcription of the ABA biosynthetic gene LjNCED2. We propose that a transcriptionally regulated, early SL decrease under osmotic stress is needed (but not sufficient) to allow the physiological increase of ABA in roots. This work shows that SL metabolism and effects on ABA are seemingly opposite in roots and shoots under stress. Springer-Verlag Berlin Heidelberg, 2015 Carotenoid cleavage enzymes nationallicence CCD7 nationallicence CCD8 nationallicence D27 nationallicence MAX1 nationallicence PDR1 nationallicence NCED nationallicence Drought nationallicence GR24 (synthetic strigolactone analogue) nationallicence Phosphate starvation nationallicence Stomatal conductance nationallicence ABA : Abscisic acid nationallicence AAO : Abscisic-aldehyde oxidase nationallicence ABC : ATP-binding cassette nationallicence AMF : Arbuscular mycorrhizal fungi nationallicence CCD : Carotenoid cleavage dioxygenase nationallicence D : Dwarf nationallicence DAD : Decreased apical dominance nationallicence EST : Expressed sequence tag nationallicence Ljccd7 : CCD7-knocked-down L. japonicus nationallicence MAX : More axillary growth nationallicence NCED : Nine-cis-epoxycarotenoid dioxygenase nationallicence PDR : Pleiotropic drug resistance nationallicence SL : Strigolactone nationallicence Liu Junwei Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut He Hanzi Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut Vitali Marco Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut Visentin Ivan Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut Charnikhova Tatsiana Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut Haider Imran Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut Schubert Andrea Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut Ruyter-Spira Carolien Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut Bouwmeester Harro Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut Lovisolo Claudio Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut Cardinale Francesca Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1435-1451 0032-0935 241:6<1435 2015 241 425 https://doi.org/10.1007/s00425-015-2266-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2266-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Junwei Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 700 1- He Hanzi Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Vitali Marco Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 700 1- Visentin Ivan Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 700 1- Charnikhova Tatsiana Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Haider Imran Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Schubert Andrea Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 700 1- Ruyter-Spira Carolien Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Bouwmeester Harro Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Lovisolo Claudio Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 700 1- Cardinale Francesca Department of Agricultural, Forest and Food Sciences (DISAFA), University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1435-1451 0032-0935 241:6<1435 2015 241 425