caa a22 4500 606208852 CHVBK 20210128101025.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00425-015-2271-y doi (NATIONALLICENCE)springer-10.1007/s00425-015-2271-y Populus endo- β -1,4-glucanases gene family: genomic organization, phylogenetic analysis, expression profiles and association mapping [Elektronische Daten] [Qingzhang Du, Lu Wang, Xiaohui Yang, Chenrui Gong, Deqiang Zhang] Main conclusion : Extensive characterization of the poplar GH9 gene family provides new insights into GH9 function and evolution in woody species, and may drive novel progress for molecular breeding in trees. Abstract: In higher plants, endo-β-1,4-glucanases (cellulases) belonging to the glycosyl hydrolase family 9 (GH9) have roles in cell wall synthesis, remodeling and degradation. To increase the understanding of the GH9 family in perennial woody species, we conducted an extensive characterization of the GH9 family in the model tree species, Populus. We characterized 25 putative GH9 members in Populus with three subclasses (A, B, and C), using structures and bioinformatic analysis. Phylogenetic analyses of 114 GH9s from plant (dicot, monocot, and conifer) and bacterial species (outgroup) demonstrated that plant GH9s are monophyletic with respect to bacteria GH9s. Three subclasses, A, B, and C, of plant GH9 are formed before the divergence of angiosperms and gymnosperms. Chromosomal localization and duplications of GH9s in the Populus genome showed that eight paralogous pairs remained in conserved positions on segmental duplicated blocks, suggesting duplication of chromosomal segments has contributed to the family expansion. By examining tissue-specific expression profiles for all 25 members, we found that GH9 members exhibited distinct but partially overlapping expression patterns, while certain members have higher transcript abundance in mature or developing xylem. Based on our understanding of intraspecific variation and linkage disequilibrium of two KORRIGANs (PtoKOR1 and PtoKOR2) in natural population of Populus tomentosa, two non-synonymous SNPs in PtoKOR1 associated with fiber width and holocellulose content were obtained. Characterizations of the poplar GH9 family provide new insights into GH9 function and evolution in woody species, and may drive novel progress for molecular breeding in trees. Springer-Verlag Berlin Heidelberg, 2015 Association mapping nationallicence Chromosomal localization nationallicence Glycosyl hydrolase family 9 nationallicence KORRIGAN nationallicence Populus nationallicence Wood properties nationallicence CBM : Carbohydrate-binding module nationallicence CSC : Cellulose synthase complex nationallicence DBH : Diameter at the breast height nationallicence ESTs : Expressed sequence tags nationallicence FDR : False discovery rate nationallicence GH9 : Glycosyl hydrolase family 9 nationallicence GLM : General linear model nationallicence INDELs : Insertions/deletions nationallicence KOR1 : KORRIGAN1 nationallicence LD : Linkage disequilibrium nationallicence LGs : Linkage groups nationallicence MFA : Minor allele frequencies nationallicence MLM : Mixed linear model nationallicence MY : Million years nationallicence NW : Northwestern nationallicence NE : Northeastern nationallicence RT-PCR : Reverse transcription PCR nationallicence RT-qPCR : Real-time quantitative PCR nationallicence r 2 : The squared correlation of allele frequencies nationallicence S : Southern subset nationallicence SNP : Single-nucleotide polymorphisms nationallicence T : Divergence time nationallicence TM : Transmembrane domain nationallicence Du Qingzhang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People's Republic of China aut Wang Lu National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People's Republic of China aut Yang Xiaohui National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People's Republic of China aut Gong Chenrui National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People's Republic of China aut Zhang Deqiang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People's Republic of China aut Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1417-1434 0032-0935 241:6<1417 2015 241 425 https://doi.org/10.1007/s00425-015-2271-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2271-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Du Qingzhang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Lu National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Yang Xiaohui National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Gong Chenrui National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Deqiang National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1417-1434 0032-0935 241:6<1417 2015 241 425