caa a22 4500 606208879 CHVBK 20210128101025.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00425-015-2270-z doi (NATIONALLICENCE)springer-10.1007/s00425-015-2270-z Genes for a series of proteins that are involved in glucose catabolism are upregulated by the Hik8-cascade in Synechocystis sp. PCC 6803 [Elektronische Daten] [Katsuhiko Okada, Eisuke Horii, Yoshiaki Nagashima, Mayuka Mitsui, Hazuki Matsuura, Shoko Fujiwara, Mikio Tsuzuki] Main conclusion : In summary, we could show the involvement of a Hik8-cascade in the expression of genes involved in the glycolytic and OPP pathways induced by GPL, and another signal pathway under photosynthetic conditions in Synechocystis . The Hik8-cascade under GPL conditions may regulate glucose degradation to produce some energy and carbon compounds. This cascade might be important for the supply of organic materials such as amino acids and nucleotides through enhancement of the rates of the glycolysis and OPP pathways. Histidine kinase Hik8 upregulates the expression of one of the important glycolytic genes, fbaA, via sll1330 under heterotrophic growth conditions (i.e., in the presence of glucose with an indispensable short period of light) in Synechocystis sp. PCC 6803. In this study, expression of the genes for the glycolytic and OPP pathways was investigated using the wild type, and disruption mutants of Hik8 and sll1330, to determine whether or not the Hik8-involving signal transduction system generally regulates glucose catabolism. In the wild type, all the genes for the glycolytic and OPP pathways were upregulated under the same conditions as for fbaA. Analyses of the disruption mutants suggested that the signal transduction system involving Hik8 and Sll1330 plays a key role in the upregulation of genes such as pfkA, pgmB, and glk, and also that Hik8 induces genes including gap1 and pgk independently of Sll1330. This complicated signal transduction cascade, designated as the Hik8-cascade, occurs under heterotrophic growth with light pulses. In addition, a disruption mutant of a putative histidine kinase, sll1334, exhibited growth and gene expression patterns that suggested it to be a negative regulator in the cascade. Possible histidine kinases and response regulators as candidates for other components in the cascade are discussed. Springer-Verlag Berlin Heidelberg, 2015 Cyanobacteria nationallicence Gene expression nationallicence Glucose metabolism nationallicence Glycolysis nationallicence Pentose phosphate pathway nationallicence Two-component system nationallicence cfxE : Gene for pentose-5-phosphate-3-epimerase nationallicence eno : Gene for enolase nationallicence FBA : Fructose 1,6-bisphosphate aldolase nationallicence fbaA : Gene for fructose-bisphosphate aldolase, class II nationallicence fbp : Gene for fructose-1,6-bisphosphatase nationallicence fda : Gene for fructose-bisphosphate aldolase, class I nationallicence gap1 : Gene for glyceraldehyde 3-phosphate dehydrogenase 1 nationallicence glk : Gene for glucokinase nationallicence gnd : Gene for 6-phosphogluconate dehydrogenase nationallicence GPL : Glucose-plus-pulsed light nationallicence hik8 : Gene for two-component sensor histidine kinase 8 nationallicence OPP pathway : Oxidative pentose phosphate pathway nationallicence pfkA : Gene for phosphofructokinase nationallicence pgi : Gene for glucose-6-phosphate isomerase nationallicence pgk : Gene for phosphoglycerate kinase nationallicence pgm : Gene for 2,3-bisphosphoglycerate-independent phosphoglycerate mutase nationallicence pgmB : Gene for phosphoglycerate mutase nationallicence pk : Gene for pyruvate kinase nationallicence rnpB : Gene for RNase P subunit B nationallicence tal : Gene for transaldolase nationallicence tktA : Gene for transketolase nationallicence zwf : Gene for glucose 6-phosphate dehydrogenase nationallicence Okada Katsuhiko School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Horii Eisuke School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Nagashima Yoshiaki School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Mitsui Mayuka School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Matsuura Hazuki School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Fujiwara Shoko School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Tsuzuki Mikio School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1453-1462 0032-0935 241:6<1453 2015 241 425 https://doi.org/10.1007/s00425-015-2270-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2270-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okada Katsuhiko School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Horii Eisuke School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Nagashima Yoshiaki School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Mitsui Mayuka School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsuura Hazuki School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Fujiwara Shoko School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 700 1- Tsuzuki Mikio School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1453-1462 0032-0935 241:6<1453 2015 241 425