caa a22 4500 606208887 CHVBK 20210128101025.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00425-015-2274-8 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2274-8 Generation of reactive oxygen species in thylakoids from senescing flag leaves of the barley varieties Lomerit and Carina [Elektronische Daten] [Anja Krieger-Liszkay, Mirl Trösch, Karin Krupinska] Main conclusion : During senescence, production of reactive oxygen species increased in thylakoids. In two barley varieties, no difference in superoxide production was observed while singlet oxygen production increased only in one variety. Abstract: During senescence, chlorophyll content decreased and photosynthetic electron transport was inhibited as shown for flag leaves collected from barley varieties Lomerit and Carina grown in the field. Spin trapping electron paramagnetic resonance (EPR) was used to investigate the production of reactive oxygen species in thylakoid membranes during senescence. EPR measurements were performed with specific spin traps to discriminate between singlet oxygen on one hand and reactive oxygen intermediates on the other hand. The results show that the generation of reactive oxygen intermediates increases in both varieties during senescence. Singlet oxygen increased only in the variety cv. Lomerit while it remained constant at a low level in the variety cv. Carina. Measurements in the presence of inhibitors of photosystem II and of the cytochrome b6f complex revealed that in senescing leaves reduction of oxygen at the acceptor side of photosystem I was the major, but not the only source of superoxide anions. This study shows that during senescence the production of individual reactive oxygen species varies in different barley varieties. Springer-Verlag Berlin Heidelberg, 2015 Spin trapping electron paramagnetic resonance nationallicence Reactive oxygen species nationallicence Thylakoids nationallicence Photosynthetic electron transport nationallicence Barley nationallicence Chl : Chlorophyll nationallicence DCMU : 3-(3,4-Dichlorophenyl)-1,1-dimethyl urea nationallicence DNP-INT : 2-Iodo-2′,4′,4′-trinitro-3-methyl-6-isopropyl diphenyl ether nationallicence EPR : Electron paramagnetic resonance nationallicence LHC : Light harvesting complex nationallicence PS : Photosystem nationallicence 4-POBN : α-(4-Pyridyl-1-oxide)-N-tert-butylnitrone nationallicence ROS : Reactive oxygen species nationallicence SOD : Superoxide dismutase nationallicence TEMPD-HCl : 2,2,6,6-Tetramethyl-4-piperidone hydrochloride nationallicence Krieger-Liszkay Anja Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA) Saclay, Institut de Biologie et de Technologie de Saclay, Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud, 91191, Gif-sur-Yvette cedex, France aut Trösch Mirl Institute of Botany, University of Kiel, Olshausenstraße 40, 24098, Kiel, Germany aut Krupinska Karin Institute of Botany, University of Kiel, Olshausenstraße 40, 24098, Kiel, Germany aut Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1497-1508 0032-0935 241:6<1497 2015 241 425 https://doi.org/10.1007/s00425-015-2274-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2274-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krieger-Liszkay Anja Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA) Saclay, Institut de Biologie et de Technologie de Saclay, Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud, 91191, Gif-sur-Yvette cedex, France aut NATIONALLICENCE 700 1- Trösch Mirl Institute of Botany, University of Kiel, Olshausenstraße 40, 24098, Kiel, Germany aut NATIONALLICENCE 700 1- Krupinska Karin Institute of Botany, University of Kiel, Olshausenstraße 40, 24098, Kiel, Germany aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1497-1508 0032-0935 241:6<1497 2015 241 425