caa a22 4500 606208895 CHVBK 20210128101025.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00425-015-2262-z doi (NATIONALLICENCE)springer-10.1007/s00425-015-2262-z Molecular characterization and transcriptome analysis of orange head Chinese cabbage ( Brassica rapa L. ssp. pekinensis ) [Elektronische Daten] [Junxiang Zhang, Hui Yuan, Zhangjun Fei, Barry Pogson, Lugang Zhang, Li Li] Main conclusion : The orange head phenotype of Br - or resulted from a large insertion incarotenoid isomerase (BrCRTISO) . Comparative transcriptome analysis revealed that the mutation affected the expression of abundant transcription factor genes. A new orange trait-specific marker was developed for marker-assisted breeding. Orange head leaves are a desirable quality trait for Chinese cabbage. Our previous fine mapping identified BrCRTISO as the Br-or candidate gene for the orange Chinese cabbage mutant. Here, we examined the BrCRTISO gene from white and orange head Chinese cabbage. While BrCRTISO from the white control plant was able to complement the Arabidopsis Atcrtiso mutant phenotype, Brcrtiso with a large insertion from the orange head Chinese cabbage failed to rescue the Arabidopsis mutant phenotype. The results show that Brcrtiso was non-functional, concomitant with the accumulation of prolycopene in Br-or to yield orange head. Comparative transcriptome analysis by RNA-seq identified 372 differentially expressed genes between the control and Br-or mutant using two near-isogenic lines with white and orange inner leaves. The mutation in BrCRTISO specifically affected many genes in the functional groups involved in RNA, protein, transport, and signaling. Particularly, expressions of many transcription factor genes were dramatically altered in Br-or, suggesting a potential role of BrCRTISO or carotenoid metabolites in affecting transcription. A novel co-dominant gene-specific marker was developed that co-segregated with orange color phenotype and would be useful for marker-assisted selection with enhanced selection efficiency. Our study provides new insights into understanding of the molecular basis of Br-or in mediating head leaf color and depicts a global view of the effect of BrCRTISO on cellular processes in plant. It also provides a molecular tool to accelerate breeding new Chinese cabbage cultivars with unique health quality and visual appearance. Springer-Verlag Berlin Heidelberg, 2015 Chinese cabbage nationallicence Orange head nationallicence BrCRTISO nationallicence Transcriptome nationallicence Gene-specific marker nationallicence MAS : Marker-assisted selection nationallicence NILs : Near-isogenic lines nationallicence GGPP : Geranylgeranyl pyrophosphate nationallicence PSY : Phytoene synthase nationallicence PDS : Phytoene desaturase nationallicence ZDS : ζ-Carotene desaturase nationallicence CRTISO : Carotenoid isomerase nationallicence LCYB : Lycopene β-cyclase nationallicence LCYE : Lycopene ε-cyclase nationallicence CHYB : β-Carotene hydroxylase nationallicence CHYE : ε-Carotene hydroxylase nationallicence ZEP : Zeaxanthin epoxidase nationallicence CCD : Carotenoid cleavage dioxygenase nationallicence NCED : 9-Cis-epoxycarotenoid dioxygenase nationallicence Zhang Junxiang College of Horticulture, State Key Laboratory of Crop Stress Biology for Arid Area, Northwest A&F University, 712100, Yangling, China aut Yuan Hui Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, 14853, Ithaca, NY, USA aut Fei Zhangjun Boyce Thompson Institute for Plant Research, Cornell University, 14853, Ithaca, NY, USA aut Pogson Barry Australian Research Council Centre of Excellence in Plant Energy Biology, The Australian National University, 0200, Canberra, ACT, Australia aut Zhang Lugang College of Horticulture, State Key Laboratory of Crop Stress Biology for Arid Area, Northwest A&F University, 712100, Yangling, China aut Li Li Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, 14853, Ithaca, NY, USA aut Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1381-1394 0032-0935 241:6<1381 2015 241 425 https://doi.org/10.1007/s00425-015-2262-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2262-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Junxiang College of Horticulture, State Key Laboratory of Crop Stress Biology for Arid Area, Northwest A&F University, 712100, Yangling, China aut NATIONALLICENCE 700 1- Yuan Hui Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Fei Zhangjun Boyce Thompson Institute for Plant Research, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Pogson Barry Australian Research Council Centre of Excellence in Plant Energy Biology, The Australian National University, 0200, Canberra, ACT, Australia aut NATIONALLICENCE 700 1- Zhang Lugang College of Horticulture, State Key Laboratory of Crop Stress Biology for Arid Area, Northwest A&F University, 712100, Yangling, China aut NATIONALLICENCE 700 1- Li Li Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1381-1394 0032-0935 241:6<1381 2015 241 425