caa a22 4500 606208925 CHVBK 20210128101025.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00425-015-2272-x doi (NATIONALLICENCE)springer-10.1007/s00425-015-2272-x Characterization of a potato activation-tagged mutant, nikku , and its partial revertant [Elektronische Daten] [Sukhwinder Aulakh, Richard Veilleux, Guozhu Tang, Barry Flinn] Main conclusion : A potato mutant with a strong stress-response phenotype, and a partial mutant revertant, were characterized. Gene expression patterns and DNA cytosine methylation varied between these and wild-type, indicating a role for DNA cytosine methylation changes in the gene expression and visible phenotypes. Abstract: Morphological and molecular studies were conducted to compare potato cv. Bintje, a Bintje activation-tagged mutant (nikku), and nikku revertant phenotype plants. Morphological studies revealed that nikku plants exhibited an extremely dwarf phenotype, had small hyponastic leaves, were rootless, and infrequently produced small tubers compared to wild-type Bintje. The overall phenotype was suggestive of a constitutive stress response, which was further supported by the greater expression level of several stress-responsive genes in nikku. Unlike the nikku mutant, the revertant exhibited near normal shoot elongation, larger leaves and consistent rooting. The reversion appeared partial, and was not the result of a loss of 35S enhancer copies from the original nikku mutant. Southern blot analyses indicated the presence of a single T-DNA insertion on chromosome 12 in the mutant. Gene expression studies comparing Bintje, nikku and revertant phenotype plants indicated transcriptional activation/repression of several genes flanking both sides of the insertion in the mutant, suggesting that activation tagging had pleiotropic effects in nikku. In contrast, gene expression levels for many, but not all, of the same genes in the revertant were similar to Bintje, indicating some reversion at the gene expression level as well. DNA methylation studies indicated differences in cytosine methylation status of the 35S enhancers between the nikku mutant and its revertant. In addition, global DNA cytosine methylation varied between Bintje, the nikku mutant and the revertant, suggesting involvement in gene expression changes, as well as mutant phenotype. Springer-Verlag Berlin Heidelberg, 2015 Activation tagging nationallicence Epigenetics nationallicence Methylation nationallicence Mutant nationallicence Solanum tuberosum nationallicence 5-mC : 5-Methylcytosine nationallicence ABA : Abscisic acid nationallicence APX : Ascorbate peroxidase nationallicence CaMV : Cauliflower mosaic virus nationallicence CBF : C-repeat binding factor nationallicence COR : Cold regulated gene nationallicence IBA : Indole-3-butyric acid nationallicence LEA : Late embryogenesis abundant nationallicence P5CS : Pyrroline-5-carboxylate synthase nationallicence RMA1H1 : RING membrane-anchor 1 homologue 1 nationallicence T-DNA : Transfer DNA nationallicence Up : Underperformer (an activation-tagged potato mutant) nationallicence WRKY : WRKY (W=Tryptophan, R=Arginine, K=Lysine, Y=Tyrosine) domain nationallicence Aulakh Sukhwinder Department of Horticulture, Virginia Tech, 24060, Blacksburg, VA, USA aut Veilleux Richard Department of Horticulture, Virginia Tech, 24060, Blacksburg, VA, USA aut Tang Guozhu Center for Sustainable and Renewable Resources (CSRR), Institute for Advanced Learning and Research (IALR), 24540, Danville, VA, USA aut Flinn Barry Department of Horticulture, Virginia Tech, 24060, Blacksburg, VA, USA aut Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1481-1495 0032-0935 241:6<1481 2015 241 425 https://doi.org/10.1007/s00425-015-2272-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2272-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aulakh Sukhwinder Department of Horticulture, Virginia Tech, 24060, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Veilleux Richard Department of Horticulture, Virginia Tech, 24060, Blacksburg, VA, USA aut NATIONALLICENCE 700 1- Tang Guozhu Center for Sustainable and Renewable Resources (CSRR), Institute for Advanced Learning and Research (IALR), 24540, Danville, VA, USA aut NATIONALLICENCE 700 1- Flinn Barry Department of Horticulture, Virginia Tech, 24060, Blacksburg, VA, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1481-1495 0032-0935 241:6<1481 2015 241 425