caa a22 4500 606208941 CHVBK 20210128101025.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00425-015-2278-4 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2278-4 The activities of four constitutively expressed promoters in single-copy transgenic rice plants for two homozygous generations [Elektronische Daten] [Seung Bang, Su-Hyun Park, Youn Kim, Yang Do Choi, Ju-Kon Kim] Main conclusion : We have characterized four novel constitutive promoters ARP1, H3F3, HSP and H2BF3 that are active in all tissues/stages of transgenic plants and stable over two homozygous generations. Gene promoters that are active and stable over several generations in transgenic plants are valuable tools for plant research and biotechnology. In this study, we characterized four putative constitutive promoters (ARP1, H3F3, HSP and H2BF3) in transgenic rice plants. Promoter regions were fused to the green fluorescence protein (GFP) reporter gene and transformed into rice. Single-copy transgenic lines were then selected and promoter activity was analyzed in various organs and tissues of two successive homozygous generations. All four promoters showed a broad expression profile in most tissues and developmental stages, and indeed the expression of the ARP1 and H3F3 promoters was even greater than that of the PGD1 promoter, a previously described constitutive promoter that has been used in transgenic rice. This observation was based on expression levels in leaves, roots, dry seeds and flowers in both the T2 and T3 generations. Each promoter exhibited comparable levels of activity over two homozygous generations with no sign of transgene silencing, which is an important characteristic of promoters to be used in crop biotechnology applications. These promoters therefore have considerable potential value for the stable and constitutive expression of transgenes in monocotyledonous crops. Springer-Verlag Berlin Heidelberg, 2015 Constitutive promoter nationallicence GFP nationallicence Homozygous generations nationallicence Oryza sativa nationallicence Single copy nationallicence Transgenic rice nationallicence ARP1 : 60S acidic ribosomal protein P1 nationallicence H2BF3 : Histone H2B.3 nationallicence H3F3 : Histone H3.3 nationallicence HSP : Heat shock protein nationallicence Bang Seung Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, 232-916, Pyeongchang, Korea aut Park Su-Hyun Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, 232-916, Pyeongchang, Korea aut Kim Youn Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, 232-916, Pyeongchang, Korea aut Do Choi Yang Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, 232-916, Pyeongchang, Korea aut Kim Ju-Kon Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, 232-916, Pyeongchang, Korea aut Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1529-1541 0032-0935 241:6<1529 2015 241 425 https://doi.org/10.1007/s00425-015-2278-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2278-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bang Seung Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, 232-916, Pyeongchang, Korea aut NATIONALLICENCE 700 1- Park Su-Hyun Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, 232-916, Pyeongchang, Korea aut NATIONALLICENCE 700 1- Kim Youn Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, 232-916, Pyeongchang, Korea aut NATIONALLICENCE 700 1- Do Choi Yang Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, 232-916, Pyeongchang, Korea aut NATIONALLICENCE 700 1- Kim Ju-Kon Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, 232-916, Pyeongchang, Korea aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1529-1541 0032-0935 241:6<1529 2015 241 425