caa a22 4500 60620895X CHVBK 20210128101025.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00425-015-2279-3 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2279-3 Unique miRNome during anthesis in drought-tolerant indica rice var. Nagina 22 [Elektronische Daten] [Shivani Kansal, Roseeta Devi, Sonia Balyan, Mukesh Arora, Anil Singh, Saloni Mathur, Saurabh Raghuvanshi] Main conclusion : Drought-tolerant rice variety, Nagina 22 (N22), has a unique spikelet miRNome during anthesis stage drought as well as transition from heading to anthesis. Molecular characterization of genetic diversity of rice is essential to understand the evolution and molecular basis of various agronomically important traits such as drought tolerance. miRNAs play an important role in regulating plant development as well as stress response such as drought. In this study, we characterized the yet unexplored dynamics of the spikelet miRNA population during developmental transition from ‘heading' to ‘anthesis' as well as anthesis stage drought stress in a drought-tolerant indica rice variety, N22. A significant proportion of miRNA population (~20%) in N22 spikelets is modulated during transition from heading to anthesis indicating a unique miRNome at anthesis, a developmental stage highly sensitive to stress (drought/heat). Based on the analysis of degradome data, majority of differentially regulated miRNAs appear to regulate transcription factors, some of which are implicated in regulation of development and fertilization. Similarly, drought during anthesis leads to a global change in miRNA expression pattern including those which regulate ROS homeostasis. It was possible to identify several miRNAs that were not reported to be drought responsive in earlier studies. Interestingly, a significant proportion of the drought-regulated miRNAs co-localize within QTLs related to drought tolerance and associated traits. Comparison of the expression profiles between N22 and Pusa Basmati 1 (drought sensitive) identified miRNAs with variety-specific expression patterns during phase transition (miR164, miR396, miR812, and miR1881) as well as drought stress (miR1881) indicating an evolution of a distinct and variety-specific regulatory mechanism. The promoters of these miRNAs contain LREs (light-responsive elements) and are induced by dark treatment. It was also possible to identify 4 novel miRNAs including an intronic miRNA that was conserved in both rice varieties. Springer-Verlag Berlin Heidelberg, 2015 Anthesis nationallicence Drought nationallicence Heading nationallicence miRNA nationallicence Rice variety Nagina 22 nationallicence N22 : Nagina 22 nationallicence PB1 : Pusa Basmati 1 nationallicence DAF : Days after fertilization nationallicence QTL : Quantitative trait loci nationallicence ROS : Reactive oxygen species nationallicence SNP : Single nucleotide polymorphism nationallicence Kansal Shivani Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut Devi Roseeta Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut Balyan Sonia Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut Arora Mukesh Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut Singh Anil Division of Genetics, Indian Agricultural Research Institute, 110012, Pusa New Delhi, India aut Mathur Saloni National Institute of Plant Genome Research, Aruna Asaf Ali Road, 110067, New Delhi, India aut Raghuvanshi Saurabh Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1543-1559 0032-0935 241:6<1543 2015 241 425 https://doi.org/10.1007/s00425-015-2279-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2279-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kansal Shivani Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Devi Roseeta Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Balyan Sonia Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Arora Mukesh Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Singh Anil Division of Genetics, Indian Agricultural Research Institute, 110012, Pusa New Delhi, India aut NATIONALLICENCE 700 1- Mathur Saloni National Institute of Plant Genome Research, Aruna Asaf Ali Road, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Raghuvanshi Saurabh Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/6(2015-06-01), 1543-1559 0032-0935 241:6<1543 2015 241 425