caa a22 4500 606208984 CHVBK 20210128101025.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00425-014-2212-1 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2212-1 Genome-wide identification of phenolic acid biosynthetic genes in Salvia miltiorrhiza [Elektronische Daten] [Bo Wang, Wei Sun, Qiushi Li, Ying Li, Hongmei Luo, Jingyuan Song, Chao Sun, Jun Qian, Yingjie Zhu, Alice Hayward, Haibin Xu, Shilin Chen] Main conclusion : Twenty-nine genes related to phenolic acid biosynthesis were identified in the Salvia miltiorrhiza genome. Nineteen of these are described for the first time, with ten genes experimentally correlating to phenolic acid biosynthesis. Vast stores of secondary metabolites exist in plants, many of which possess biological activities related to human health. Phenolic acid derivatives are a class of valuable bioactive pharmaceuticals abundant in the widely used Chinese medicinal herb, Salvia miltiorrhiza. The biosynthetic pathway for phenolic acids differs in this species from that of other investigated plants. However, the molecular basis for this is unknown, with systematic analysis of the genes involved not yet performed. As the first step towards unraveling this complex biosynthetic pathway in S. miltiorrhiza, the current genome assembly was searched for putatively involved genes. Twenty-nine genes were revealed, 19 of which are described here for the first time. These include 15 genes predicted in the phenylpropanoid pathway; seven genes in the tyrosine-derived pathway; six genes encoding putative hydroxycinnamoyltransferases, and one CYP98A, namely CYP98A78. The promoter regions, gene structures and expression patterns of these genes were examined. Furthermore, conserved domains and phylogenetic relationships with homologous proteins in other species were revealed. Most of the key enzymes, including 4-coumarate: CoA ligase, 4-hydroxyphenylpyruvate reductase and hydroxycinnamoyltransferase, were found in multiple copies, each exhibiting different characteristics. Ten genes putatively involved in rosmarinic acid biosynthesis are also described. These findings provide a foundation for further analysis of this complex and diverse pathway, with potential to enhance the synthesis of water-soluble medicinal compounds in S. miltiorrhiza. Springer-Verlag Berlin Heidelberg, 2014 Phenolic acids nationallicence Phenolic acid biosynthesis nationallicence Rosmarinic acid nationallicence Medicinal compounds nationallicence DHPL : 3,4-Dihydroxyphenyllactate nationallicence pHPL : 4-Hydroxyphenyllactate nationallicence RA : Rosmarinic acid nationallicence TAT : Tyrosine aminotransferase nationallicence HCT : Hydroxycinnamoyltransferase nationallicence RAS : Rosmarinic acid synthase=hydroxycinnamoyl-CoA:hydroxyphenyllactate HCT nationallicence HPPR : 4-Hydroxyphenylpyruvate reductase nationallicence C4H : Cinnamate 4-hydroxylase nationallicence HPPD : 4-Hydroxyphenylpyruvate dioxygenase nationallicence PAL : Phenylalanine ammonia-lyase nationallicence 4CL : 4-Coumarate:CoA ligase nationallicence Wang Bo Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut Sun Wei Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, 100700, Beijing, China aut Li Qiushi Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut Li Ying Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut Luo Hongmei Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut Song Jingyuan Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut Sun Chao Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut Qian Jun Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut Zhu Yingjie Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut Hayward Alice Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 4072, Brisbane, Australia aut Xu Haibin Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut Chen Shilin Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut Planta Springer Berlin Heidelberg 241/3(2015-03-01), 711-725 0032-0935 241:3<711 2015 241 425 https://doi.org/10.1007/s00425-014-2212-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2212-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Bo Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut NATIONALLICENCE 700 1- Sun Wei Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, 100700, Beijing, China aut NATIONALLICENCE 700 1- Li Qiushi Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut NATIONALLICENCE 700 1- Li Ying Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut NATIONALLICENCE 700 1- Luo Hongmei Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut NATIONALLICENCE 700 1- Song Jingyuan Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut NATIONALLICENCE 700 1- Sun Chao Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut NATIONALLICENCE 700 1- Qian Jun Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut NATIONALLICENCE 700 1- Zhu Yingjie Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut NATIONALLICENCE 700 1- Hayward Alice Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 4072, Brisbane, Australia aut NATIONALLICENCE 700 1- Xu Haibin Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut NATIONALLICENCE 700 1- Chen Shilin Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100193, Beijing, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/3(2015-03-01), 711-725 0032-0935 241:3<711 2015 241 425