caa a22 4500 60620900X CHVBK 20210128101026.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00425-014-2208-x doi (NATIONALLICENCE)springer-10.1007/s00425-014-2208-x Shoot-derived signals other than auxin are involved in systemic regulation of strigolactone production in roots [Elektronische Daten] [Kaori Yoneyama, Takaya Kisugi, Xiaonan Xie, Ryota Arakawa, Tatsuhiro Ezawa, Takahito Nomura, Koichi Yoneyama] Main conclusion : Nitrogen and phosphorus fertilization in one side of split-root sorghum plants systemically reduced root contents of strigolactones in both sides of the split roots. Shoot-derived signals other than auxin appeared to be involved in this process. Strigolactones (SLs) are a novel class of plant hormones regulating both shoot and root architectures and suggested to be functioning downstream of auxin. The levels of SLs in plant tissues and root exudates are regulated by nutrients, especially phosphorus (P) and nitrogen (N); however, the underlying mechanism remains elusive. We examined the effects of N and P fertilization on root contents of two SLs, sorgomol and 5-deoxystrigol, in sorghum plants pre-incubated under N and P free conditions using a split-root system. N and P fertilization to one side of the split-root plants systemically reduced root contents of SLs in both sides of the split roots. The shoot N and P levels increased when one side of the split-root plants was fertilized, while N and P levels in the non-fertilized split roots were unaffected. N fertilization decreased shoot and root IAA (indole-3-acetic acid) levels, while P fertilization did not affect them. IAA applied to the shoot apices increased root contents of 5-deoxystrigol but not that of sorgomol only when the plants were grown under P free conditions. Shoot (leaf) removal dramatically decreased the root contents of SLs but did not affect root IAA levels, and IAA applied to the stumps of leaves could not restore root contents of SLs. Consequently, shoot-derived signals other than auxin are suggested to be involved in the regulation of SL production in roots. Springer-Verlag Berlin Heidelberg, 2014 Auxin transport inhibitor nationallicence Indole-3-acetic acid nationallicence Nitrogen deficiency nationallicence Phosphorus deficiency nationallicence Shoot-derived signal nationallicence Sorghum nationallicence Strigolactone nationallicence AM : Arbuscular mycorrhizal nationallicence HPLC : High performance liquid chromatography nationallicence IAA : Indole-3-acetic acid nationallicence LC-MS/MS : Liquid chromatography/tandem mass spectrometry nationallicence NPA : N-naphthylphthalamic acid nationallicence SL : Strigolactone nationallicence Yoneyama Kaori Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut Kisugi Takaya Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut Xie Xiaonan Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut Arakawa Ryota Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan aut Ezawa Tatsuhiro Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan aut Nomura Takahito Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut Yoneyama Koichi Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut Planta Springer Berlin Heidelberg 241/3(2015-03-01), 687-698 0032-0935 241:3<687 2015 241 425 https://doi.org/10.1007/s00425-014-2208-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2208-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoneyama Kaori Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut NATIONALLICENCE 700 1- Kisugi Takaya Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut NATIONALLICENCE 700 1- Xie Xiaonan Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut NATIONALLICENCE 700 1- Arakawa Ryota Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan aut NATIONALLICENCE 700 1- Ezawa Tatsuhiro Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan aut NATIONALLICENCE 700 1- Nomura Takahito Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut NATIONALLICENCE 700 1- Yoneyama Koichi Center for Bioscience Research and Education, Utsunomiya University, 350 Mine-machi, 321-8505, Utsunomiya, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/3(2015-03-01), 687-698 0032-0935 241:3<687 2015 241 425