caa a22 4500 606209026 CHVBK 20210128101026.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00425-014-2205-0 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2205-0 Genomic origin, expression differentiation and regulation of multiple genes encoding CYP83A1, a key enzyme for core glucosinolate biosynthesis, from the allotetraploid Brassica juncea [Elektronische Daten] [Meenu,, Rehna Augustine, Manoj Majee, Akshay Pradhan, Naveen Bisht] Main conclusion : The multiple BjuCYP83A1 genes formed as a result of polyploidy have retained cell-, tissue-, and condition-specific transcriptional sub-functionalization to control the complex aliphatic glucosinolates biosynthesis in the allotetraploid Brassica juncea. Glucosinolates along with their breakdown products are associated with diverse roles in plant metabolism, plant defense and animal nutrition. CYP83A1 is a key enzyme that oxidizes aliphatic aldoximes to aci-nitro compounds in the complex aliphatic glucosinolate biosynthetic pathway. In this study, we reported the isolation of four CYP83A1 genes named BjuCYP83A1-1, -2, -3, and -4 from allotetraploid Brassica juncea (AABB genome), an economically important oilseed crop of Brassica genus. The deduced BjuCYP83A1 proteins shared 85.7-88.4% of sequence identity with A. thaliana AtCYP83A1 and 84.2-95.8% among themselves. Phylogenetic and divergence analysis revealed that the four BjuCYP83A1 proteins are evolutionary conserved and have evolved via duplication and hybridization of two relatively simpler diploid Brassica genomes namely B. rapa (AA genome) and B. nigra (BB genome), and have retained high level of sequence conservation following allopolyploidization. Ectopic over-expression of BjuCYP83A1-1 in A. thaliana showed that it is involved mainly in the synthesis of C4 aliphatic glucosinolates. Detailed expression analysis using real-time qRT-PCR in B. juncea and PromoterBjuCYP83A1-GUS lines in A. thaliana confirmed that the four BjuCYP83A1 genes have retained ubiquitous, overlapping but distinct expression profiles in different tissue and cell types of B. juncea, and in response to various elicitor treatments and environmental conditions. Taken together, this study demonstrated that transcriptional sub-functionalization and coordinated roles of multiple BjuCYP83A1 genes control the biosynthesis of aliphatic glucosinolates in the allotetraploid B. juncea, and provide a framework for metabolic engineering of aliphatic glucosinolates in economically important Brassica species. Springer-Verlag Berlin Heidelberg, 2014 Brassica nationallicence CYP83A1 nationallicence Gene expression nationallicence Glucosinolate nationallicence Sub-functionalization nationallicence CYP : Cytochrome P450 nationallicence DW : Dry weight nationallicence GSL : Glucosinolate (s) nationallicence GUS : β-Glucuronidase nationallicence mya : Million years ago nationallicence Meenu, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 110067, New Delhi, India aut Augustine Rehna National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 110067, New Delhi, India aut Majee Manoj National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 110067, New Delhi, India aut Pradhan Akshay Department of Genetics, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut Bisht Naveen National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 110067, New Delhi, India aut Planta Springer Berlin Heidelberg 241/3(2015-03-01), 651-665 0032-0935 241:3<651 2015 241 425 https://doi.org/10.1007/s00425-014-2205-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2205-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meenu National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Augustine Rehna National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Majee Manoj National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Pradhan Akshay Department of Genetics, University of Delhi South Campus, Benito Juarez Marg, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Bisht Naveen National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 110067, New Delhi, India aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/3(2015-03-01), 651-665 0032-0935 241:3<651 2015 241 425