caa a22 4500 606209050 CHVBK 20210128101026.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00425-014-2214-z doi (NATIONALLICENCE)springer-10.1007/s00425-014-2214-z Over-expression of a glutamate dehydrogenase gene, MgGDH , from Magnaporthe grisea confers tolerance to dehydration stress in transgenic rice [Elektronische Daten] [Yanbiao Zhou, Caisheng Zhang, Jianzhong Lin, Yuanzhu Yang, Yuchong Peng, Dongying Tang, Xiaoying Zhao, Yonghua Zhu, Xuanming Liu] Main conclusion : Heterologous expression of a fungal NADP(H)-GDH gene ( MgGDH ) from Magnaporthe grisea can improve dehydration stress tolerance in rice by preventing toxic accumulation of ammonium. Glutamate dehydrogenase (GDH; EC 1.4.1.2 and EC 1.4.1.4) may act as a stress-responsive enzyme in detoxification of high intracellular ammonia and production of glutamate for proline synthesis under stress conditions. In present study, a fungal NADP(H)-GDH gene (MgGDH) from Magnaporthe grisea was over-expressed in rice (Oryza sativa L. cv. ‘kitaake'), and the transgenic plants showed the improvement of tolerance to dehydration stress. The kinetic analysis showed that His-TF-MgGDH preferentially utilizes ammonium to produce l-glutamate. Moreover, the affinity of His-TF-MgGDH for ammonium was dramatically higher than that of His-TF-OsGDH for ammonium. Over-expressing MgGDH transgenic rice plants showed lower water-loss rate and higher completely close stomata than the wild-type plants under dehydration stress conditions. In transgenic plants, the NADP(H)-GDH activities were markedly higher than those in wild-type plants and the amination activity was significantly higher than the deamination activity. Compared with wild-type plants, the transgenic plants accumulated much less NH4 + but higher amounts of glutamate, proline and soluble sugar under dehydration stress conditions. These results indicate that heterologous expression of MgGDH can prevent toxic accumulation of ammonium and in return improve dehydration stress tolerance in rice. Springer-Verlag Berlin Heidelberg, 2014 Rice nationallicence Magnaporthe grisea nationallicence NADP(H)-dependent glutamate dehydrogenase nationallicence Dehydration stress nationallicence Mg : Magnaporthe grisea nationallicence GDH : Glutamate dehydrogenase nationallicence GOGAT : Glutamate synthase nationallicence GS : Glutamine synthetase nationallicence 2-OG : 2-oxoglutarate nationallicence TF : Trigger factor nationallicence P5CS : △1-Pyrroline-5-carboxylate synthetase nationallicence P5CR : △1-Pyrroline-5-carboxylic acid reductase nationallicence GSA : Glutamic γ-semialdehyde nationallicence PCR : Polymerase chain reaction nationallicence Zhou Yanbiao Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut Zhang Caisheng Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut Lin Jianzhong Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut Yang Yuanzhu Academy of Seed Industry of Hunan Yahua, 410001, Changsha, Hunan, China aut Peng Yuchong Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut Tang Dongying Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut Zhao Xiaoying Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut Zhu Yonghua Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut Liu Xuanming Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut Planta Springer Berlin Heidelberg 241/3(2015-03-01), 727-740 0032-0935 241:3<727 2015 241 425 https://doi.org/10.1007/s00425-014-2214-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2214-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Yanbiao Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Zhang Caisheng Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Lin Jianzhong Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Yang Yuanzhu Academy of Seed Industry of Hunan Yahua, 410001, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Peng Yuchong Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Tang Dongying Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Zhao Xiaoying Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Zhu Yonghua Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 700 1- Liu Xuanming Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, 410082, Changsha, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/3(2015-03-01), 727-740 0032-0935 241:3<727 2015 241 425