caa a22 4500 606209077 CHVBK 20210128101026.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00425-014-2211-2 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2211-2 Disruption of prefoldin-2 protein synthesis in root-knot nematodes via host-mediated gene silencing efficiently reduces nematode numbers and thus protects plants [Elektronische Daten] [Hemavathi Ajjappala, Ha Chung, Joon-Soo Sim, Inchan Choi, Bum-Soo Hahn] Main conclusion : The aim of this study is to demonstrate the feasibility of down-regulating endogeneous prefoldin-2 root-knot nematode transcripts by expressing dsRNA with sequence identity to the nematode gene in tobacco roots under the influence of strong Arabidopsis ubiquitin (UBQ1) promoter. Root-knot nematodes (RKNs) are sedentary endoparasites infecting a wide range of plant species. They parasitise the root system, thereby disrupting water and nutrient uptake and causing major reductions in crop yields. The most reliable means of controlling RKNs is via the use of soil fumigants such as methyl bromide. With the emergence of RNA interference (RNAi) technology, which permits host-mediated nematode gene silencing, a new strategy to control plant pathogens has become available. In the present study, we investigated host-induced RNAi gene silencing of prefoldin-2 in transgenic Nicotiana benthamiana. Reductions in prefoldin-2 mRNA transcript levels were observed when nematodes were soaked in a dsRNA solution in vitro. Furthermore, nematode reproduction was suppressed in RNAi transgenic lines, as evident by reductions in the numbers of root knots (by 34-60% in independent RNAi lines) and egg masses (by 33-58%). Endogenous expression of prefoldin-2, analysed via real-time polymerase chain reaction and Western blotting, revealed that the gene was strongly expressed in the pre-parasitic J2 stage. Our observations demonstrate the relevance and potential importance of targeting the prefoldin gene during the nematode life cycle. The work also suggests that further improvements in silencing efficiency in economically important crops can be accomplished using RNAi directed against plant-parasitic nematodes. Springer-Verlag Berlin Heidelberg, 2014 Meloidogyne incognita nationallicence Plant-parasitic nematodes nationallicence Prefoldin -2 gene nationallicence RNA interference nationallicence Root-knot nematode nationallicence dsRNA : Double-strand RNA nationallicence GUS : β-Glucuronidase nationallicence J2 : Juvenile 2 nationallicence PFD : Prefoldin nationallicence RKN : Root-knot nematode nationallicence RNAi : RNA interference nationallicence RT-PCR : Reverse transcription-polymerase chain reaction nationallicence UBQ : Ubiquitin nationallicence Ajjappala Hemavathi National Academy of Agricultural Science, 441-857, Suwon, Korea aut Chung Ha National Academy of Agricultural Science, 441-857, Suwon, Korea aut Sim Joon-Soo National Academy of Agricultural Science, 441-857, Suwon, Korea aut Choi Inchan National Academy of Agricultural Science, 441-857, Suwon, Korea aut Hahn Bum-Soo National Academy of Agricultural Science, 441-857, Suwon, Korea aut Planta Springer Berlin Heidelberg 241/3(2015-03-01), 773-787 0032-0935 241:3<773 2015 241 425 https://doi.org/10.1007/s00425-014-2211-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2211-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ajjappala Hemavathi National Academy of Agricultural Science, 441-857, Suwon, Korea aut NATIONALLICENCE 700 1- Chung Ha National Academy of Agricultural Science, 441-857, Suwon, Korea aut NATIONALLICENCE 700 1- Sim Joon-Soo National Academy of Agricultural Science, 441-857, Suwon, Korea aut NATIONALLICENCE 700 1- Choi Inchan National Academy of Agricultural Science, 441-857, Suwon, Korea aut NATIONALLICENCE 700 1- Hahn Bum-Soo National Academy of Agricultural Science, 441-857, Suwon, Korea aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/3(2015-03-01), 773-787 0032-0935 241:3<773 2015 241 425