caa a22 4500 606209085 CHVBK 20210128101026.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00425-014-2215-y doi (NATIONALLICENCE)springer-10.1007/s00425-014-2215-y Identification and phylogenetic analysis of late embryogenesis abundant proteins family in tomato ( Solanum lycopersicum ) [Elektronische Daten] [Jun Cao, Xiang Li] Main conclusion : This study provided a comparative genomic analysis of the LEA gene family, and these may provide valuable information for their functional investigations in the future. Late embryogenesis abundant (LEA) proteins are a group of proteins that accumulate in response to cellular dehydration in many organisms. Here, we identified 27 LEA genes in tomato. A strong correlation between phylogeny, gene structure, and motif composition was found. The predicted SlLEA genes were non-randomly distributed within their chromosomes, and segmental and tandem duplications were probably important for their expansion. Many cis-elements potentially mediating transcription in response to abiotic stress were also found in the 1,000bp upstream sequence of the promoter region. An additional intragenic recombination played an important role in the evolution of SlLEA genes. Selection analysis also identified some significant site-specific constraints that acted on the evolution of most LEA paralogs. Expression analysis using both microarray data and quantitative real-time PCR indicated that SlLEA genes were widely expressed in various tissues, and that a few members responded to some abiotic stresses. Our study provides useful information on the LEA genes in tomato and will facilitate their further characterization to better understand their functions. Springer-Verlag Berlin Heidelberg, 2014 Expression profile nationallicence LEA nationallicence Phylogenetic analysis nationallicence Recombination nationallicence Selective pressure nationallicence Tomato nationallicence LEA : Late embryogenesis abundant nationallicence MEME : Multiple EM for Motif Elicitation nationallicence MRCA : The most recent common ancestor nationallicence NJ : Neighbor joining nationallicence qRT-PCR : Quantitative real-time polymerase chain reaction nationallicence TSS : Transcription start site nationallicence Cao Jun Institute of Life Science, Jiangsu University, 212013, Zhenjiang, Jiangsu, People's Republic of China aut Li Xiang Institute of Life Science, Jiangsu University, 212013, Zhenjiang, Jiangsu, People's Republic of China aut Planta Springer Berlin Heidelberg 241/3(2015-03-01), 757-772 0032-0935 241:3<757 2015 241 425 https://doi.org/10.1007/s00425-014-2215-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2215-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cao Jun Institute of Life Science, Jiangsu University, 212013, Zhenjiang, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Li Xiang Institute of Life Science, Jiangsu University, 212013, Zhenjiang, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/3(2015-03-01), 757-772 0032-0935 241:3<757 2015 241 425