caa a22 4500 606209107 CHVBK 20210128101026.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00425-014-2201-4 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2201-4 Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain [Elektronische Daten] [Richard Palmer, Valérie Cornuault, Susan Marcus, J. Knox, Peter Shewry, Paola Tosi] Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre. Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain. Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes. Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling. A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls. Callose has been reported to be associated with the formation of cell wall outgrowths during endosperm cellularisation and xyloglucan is here shown to be a component of these anticlinal extensions, occurring transiently in both species. Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form. A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain. Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues. In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity. The Author(s), 2014 Cell wall nationallicence Grain development nationallicence Immunodetection nationallicence Rice grain nationallicence Wheat grain nationallicence AX : Arabinoxylan nationallicence DAA : Days after anthesis nationallicence FT-IR : Fourier transform infrared spectroscopy nationallicence GalA : Galacturonic acid nationallicence GUX : Glucuronoxylan nationallicence HG : Homogalacturonan nationallicence MLG : Mixed-linkage glucan nationallicence NP : Nucellar projection nationallicence PBS : Phosphate buffer saline nationallicence RG-I : Rhamnogalacturonan-I nationallicence RG-II : Rhamnogalacturonan-II nationallicence RT : Room temperature nationallicence SE : Starchy endosperm nationallicence TS : Transverse section nationallicence VB : Vascular bundle nationallicence Palmer Richard Rothamsted Research, AL5 2JQ, Harpenden, UK aut Cornuault Valérie Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Marcus Susan Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Knox J. Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Shewry Peter Rothamsted Research, AL5 2JQ, Harpenden, UK aut Tosi Paola Rothamsted Research, AL5 2JQ, Harpenden, UK aut Planta Springer Berlin Heidelberg 241/3(2015-03-01), 669-685 0032-0935 241:3<669 2015 241 425 https://doi.org/10.1007/s00425-014-2201-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2201-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Palmer Richard Rothamsted Research, AL5 2JQ, Harpenden, UK aut NATIONALLICENCE 700 1- Cornuault Valérie Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Marcus Susan Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Knox J. Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Shewry Peter Rothamsted Research, AL5 2JQ, Harpenden, UK aut NATIONALLICENCE 700 1- Tosi Paola Rothamsted Research, AL5 2JQ, Harpenden, UK aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/3(2015-03-01), 669-685 0032-0935 241:3<669 2015 241 425