caa a22 4500 60620914X CHVBK 20210128101026.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00425-014-2204-1 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2204-1 AtrbohD and AtrbohF negatively regulate lateral root development by changing the localized accumulation of superoxide in primary roots of Arabidopsis [Elektronische Daten] [Ning Li, Lirong Sun, Liyue Zhang, Yalin Song, Panpan Hu, Cui Li, Fu Hao] Main conclusion : NADPH oxidase AtrbohD an d AtrbohF negatively modulate lateral root development by changing the peroxidase activity and increasing the local generation of superoxide in primary roots of Arabidopsis in an auxin-independent manner. NADPH oxidase subunits AtrbohD and AtrbohF play pivotal roles in regulating growth, development and stress responses in Arabidopsis. However, whether they modulate lateral root (LR) formation has not yet been addressed, and the detailed mechanisms underlying the process remain unanswered. Here, we show that two null double mutants atrbohD1/F1 and atrbohD2/F2, in which both AtrbohD and AtrbohF genes are disrupted, had remarkably higher LR density than wild-type (WT), or the single mutant atrbohD1 and atrbohF1. Compared to WT, the double mutants exhibited early emerged LRs and enhanced density of lateral root primordia (LRP). Unexpectedly, the production of superoxide (O2 −), but not hydrogen peroxide, in the mature area of the primary root containing LRs significantly increased in the double mutants relative to that in WT. Further experiments revealed that the local accumulation of O2 − led to the enhancement of LR density in the double mutants. Moreover, the deficiency of AtrbohD and AtrbohF caused a marked increase in peroxidase activity in the mature root zone, which contributed to the localized accumulation of O2 − and the elevated LR density in the double mutants. Furthermore, the double mutants were not sensitive to exogenous auxin naphthalene acetic acid or auxin transport inhibitor 1-N-naphthylphthalamic acid in terms of LR formation. The auxin response of LRP in vivo in atrbohD1/F1 was also similar to that in WT. Taken together, these results suggest that AtrbohD and AtrbohF negatively modulate LR development by controlling the local generation of superoxide in an auxin-independent manner. These findings provide new insights into the mechanisms of NADPH oxidase-mediated regulation of LR branching in Arabidopsis. Springer-Verlag Berlin Heidelberg, 2014 Auxin nationallicence Lateral root formation nationallicence NADPH oxidase nationallicence Peroxidase nationallicence Superoxide nationallicence LRs : Lateral roots nationallicence LRP : Lateral root primordia nationallicence ROS : Reactive oxygen species nationallicence MS : Murashige-Skoog nationallicence MV : Methyl viologen nationallicence RB : Rose Bengal nationallicence 4-OH-TEMPO : 4-Hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl nationallicence SHAM : Salicylhydroxamic acid nationallicence H2DCFDA 2 : 7-Dichlorodihydrofluorescein diacetate nationallicence DHE : Dihydroethidium nationallicence DAB : 3, 5-Diaminobenzidine nationallicence NBT : Nitroblue tetrazolium nationallicence NPA : 1-N-naphthylphthalamic acid nationallicence NAA : Naphthalene acetic acid nationallicence dpg : Day postgermination nationallicence Li Ning State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut Sun Lirong State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut Zhang Liyue State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut Song Yalin State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut Hu Panpan State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut Li Cui State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut Hao Fu State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut Planta Springer Berlin Heidelberg 241/3(2015-03-01), 591-602 0032-0935 241:3<591 2015 241 425 https://doi.org/10.1007/s00425-014-2204-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2204-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Ning State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut NATIONALLICENCE 700 1- Sun Lirong State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut NATIONALLICENCE 700 1- Zhang Liyue State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut NATIONALLICENCE 700 1- Song Yalin State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut NATIONALLICENCE 700 1- Hu Panpan State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut NATIONALLICENCE 700 1- Li Cui State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut NATIONALLICENCE 700 1- Hao Fu State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Jinming Street, 475004, Kaifeng, Henan, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/3(2015-03-01), 591-602 0032-0935 241:3<591 2015 241 425