caa a22 4500 606209166 CHVBK 20210128101027.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00425-014-2225-9 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2225-9 ERF022 impacts the induction of somatic embryogenesis in Arabidopsis through the ethylene-related pathway [Elektronische Daten] [Katarzyna Nowak, Barbara Wójcikowska, Małgorzata Gaj] Main conclusion : The ERF022 gene was found to affect embryogenic transition in somatic cells in Arabidopsis via the ethylene-related pathway. The study provides evidence that ERF022 - LEC2 interaction is involved in the auxin-ethylene crosstalk that operates in somatic embryogenesis induction. The ERF022 gene of the ERF family was previously identified among the transcription factor genes that were differentially expressed in an embryogenic culture of Arabidopsis. A strong inhibition of the gene was found to be associated with the induction of somatic embryogenesis (SE) and an erf022 mutant was indicated to display a substantially impaired capacity for SE. Therefore, the molecular function of ERF022 in the induction of SE was studied in the present work. A phenotype of an erf022 mutant was indicated as being related to an increased content of ethylene. The results further suggest that the ERF022 controls the genes that are involved in both the biosynthesis (ACS7) and signalling (ERF1, ETR1) of ethylene and indicate that the ERF022 is a new regulatory element in ethylene-related responses that negatively control the ethylene content and perception. It is proposed that the negative impact of ethylene on the induction of SE may result from a modulation of the auxin-related genes that control the embryogenic transition in somatic cells. Among them, the LEC2, which is a key regulator of the induction of SE through the stimulation of auxin synthesis, was possibly related to ERF022. The results of the study provide new hormone-related clues to define the genetic network that governs SE. A putative model of the regulatory pathway is proposed that is involved in the induction of SE in which the auxin-ethylene interactions are controlled by ERF022 and LEC2 and their targets. The Author(s), 2014 Auxin nationallicence Ethylene nationallicence ETHYLENE RESPONSE FACTOR022 nationallicence LEAFY COTYLEDON2 nationallicence Somatic embryogenesis nationallicence YUCCA genes nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence 2iP : 6-(a,a-Dimethylallylamino)-purine nationallicence ACC : 1-Aminocyclopropane-1-carboxylic acid nationallicence AVG : Aminoethoxyvinylglycine nationallicence BAP : 6-Benzylaminopurine nationallicence CIM : Callus induction medium nationallicence Ct : Threshold cycle nationallicence E5 : Embryogenesis induction medium with auxin nationallicence E0 : Auxin-free induction medium nationallicence IAA : Indole-3-acetic acid nationallicence IZE : Immature zygotic embryo nationallicence JA : Jasmonic acid nationallicence MeJA : Methyl jasmonate nationallicence NAA : 1-Naphthaleneacetic acid nationallicence ORG : Shoot organogenesis nationallicence SE : Somatic embryogenesis nationallicence SIM : Shoot induction medium nationallicence TF : Transcription factor nationallicence Nowak Katarzyna Department of Genetics, University of Silesia, Jagiellonska 28, 40-032, Katowice, Poland aut Wójcikowska Barbara Department of Genetics, University of Silesia, Jagiellonska 28, 40-032, Katowice, Poland aut Gaj Małgorzata Department of Genetics, University of Silesia, Jagiellonska 28, 40-032, Katowice, Poland aut Planta Springer Berlin Heidelberg 241/4(2015-04-01), 967-985 0032-0935 241:4<967 2015 241 425 https://doi.org/10.1007/s00425-014-2225-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2225-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nowak Katarzyna Department of Genetics, University of Silesia, Jagiellonska 28, 40-032, Katowice, Poland aut NATIONALLICENCE 700 1- Wójcikowska Barbara Department of Genetics, University of Silesia, Jagiellonska 28, 40-032, Katowice, Poland aut NATIONALLICENCE 700 1- Gaj Małgorzata Department of Genetics, University of Silesia, Jagiellonska 28, 40-032, Katowice, Poland aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/4(2015-04-01), 967-985 0032-0935 241:4<967 2015 241 425