caa a22 4500 606209174 CHVBK 20210128101027.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00425-014-2237-5 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2237-5 The application of Arabidopsis thaliana in studying tripartite interactions among plants, beneficial fungal endophytes and biotrophic plant-parasitic nematodes [Elektronische Daten] [Alfonso Martinuz, Getaneh Zewdu, Nicole Ludwig, Florian Grundler, Richard Sikora, Alexander Schouten] Main conclusion : The research demonstrated that Arabidopsis can be used as a model system for studying plant-nematode-endophyte tripartite interactions; thus, opening new possibilities for further characterizing the molecular mechanisms behind these interactions. Arabidopsis has been established as an important model system for studying plant biology and plant-microbe interactions. We show that this plant can also be used for studying the tripartite interactions among plants, the root-knot nematode Meloidogyne incognita and a beneficial endophytic isolate of Fusarium oxysporum, strain Fo162. In various plant species, Fo162 can systemically reduce M. incognita infection development and fecundity. Here it is shown that Fo162 can also colonize A. thaliana roots without causing disease symptoms, thus behaving as a typical endophyte. As observed for other plants, this endophyte could not migrate from the roots into the shoots and leaves. Direct inoculation of the leaves also did not result in colonization of the plant. A significant increase in plant fresh weight, root length and average root diameter was observed, suggesting the promotion of plant growth by the endophyte. The inoculation of A. thaliana with F. oxysporum strain Fo162 also resulted in a significant reduction in the number of M. incognita juveniles infecting the roots and ultimately the number of galls produced. This was also observed in a split-root experiment, in which the endophyte and nematode were spatially separated. The usefulness of Arabidopsis opens new possibilities for further dissecting complex tripartite interactions at the molecular and biochemical level. Springer-Verlag Berlin Heidelberg, 2014 Biotrophic plant-parasitic nematodes nationallicence Endophyte nationallicence Growth promotion nationallicence Model system nationallicence Tripartite interactions nationallicence AGC2-2 : Protein kinase of the AGC2 subfamily nationallicence Fo162 : Fusarium oxysporum strain Fo162 nationallicence H2O2 : Hydrogen peroxide nationallicence J2 : Infective second-stage juveniles of Meloidogyne incognita nationallicence NaOCl : Sodium hypochloride nationallicence PA : Phosphatidic acid nationallicence PDK1 : 3-Phosphoinositide-dependent protein kinase1 nationallicence OXI1 : Oxidative signal-inducible1 protein kinase nationallicence Martinuz Alfonso INRES-Soil Ecosystem Phytopathology and Nematology, University of Bonn, Nussallee 9, 53115, Bonn, Germany aut Zewdu Getaneh INRES-Molecular Phytomedicine, University of Bonn, Karlrobert-Kreiten-Straße 13, 53115, Bonn, Germany aut Ludwig Nicole INRES-Molecular Phytomedicine, University of Bonn, Karlrobert-Kreiten-Straße 13, 53115, Bonn, Germany aut Grundler Florian INRES-Molecular Phytomedicine, University of Bonn, Karlrobert-Kreiten-Straße 13, 53115, Bonn, Germany aut Sikora Richard INRES-Soil Ecosystem Phytopathology and Nematology, University of Bonn, Nussallee 9, 53115, Bonn, Germany aut Schouten Alexander INRES-Molecular Phytomedicine, University of Bonn, Karlrobert-Kreiten-Straße 13, 53115, Bonn, Germany aut Planta Springer Berlin Heidelberg 241/4(2015-04-01), 1015-1025 0032-0935 241:4<1015 2015 241 425 https://doi.org/10.1007/s00425-014-2237-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2237-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martinuz Alfonso INRES-Soil Ecosystem Phytopathology and Nematology, University of Bonn, Nussallee 9, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Zewdu Getaneh INRES-Molecular Phytomedicine, University of Bonn, Karlrobert-Kreiten-Straße 13, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Ludwig Nicole INRES-Molecular Phytomedicine, University of Bonn, Karlrobert-Kreiten-Straße 13, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Grundler Florian INRES-Molecular Phytomedicine, University of Bonn, Karlrobert-Kreiten-Straße 13, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Sikora Richard INRES-Soil Ecosystem Phytopathology and Nematology, University of Bonn, Nussallee 9, 53115, Bonn, Germany aut NATIONALLICENCE 700 1- Schouten Alexander INRES-Molecular Phytomedicine, University of Bonn, Karlrobert-Kreiten-Straße 13, 53115, Bonn, Germany aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/4(2015-04-01), 1015-1025 0032-0935 241:4<1015 2015 241 425