caa a22 4500 606209204 CHVBK 20210128101027.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00425-014-2220-1 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2220-1 Effect of silicon on wheat seedlings ( Triticum turgidum L.) grown in hydroponics and exposed to 0 to 30µM Cu [Elektronische Daten] [C. Keller, M. Rizwan, J.-C. Davidian, O. Pokrovsky, N. Bovet, P. Chaurand, J.-D. Meunier] Main conclusion : Aqueous Si limits Cu uptake by a Si-accumulating plant via physicochemical mechanisms occurring at the root level. Sufficient Si supply may alleviate Cu toxicity in Cu-contaminated soils. Little information is available on the role of silicon (Si) in copper (Cu) tolerance while Cu toxicity is widespread in crops grown on Cu-contaminated soils. A hydroponic study was set up to investigate the influence of Si on Cu tolerance in durum wheat (Triticum turgidum L.) grown in 0, 0.7, 7.0 and 30µM Cu without and with 1.0mM Si, and to identify the mechanisms involved in mitigation of Cu toxicity. Si supply alleviated Cu toxicity in durum wheat at 30µM Cu, while Cu significantly increased Si concentration in roots. Root length, photosynthetic pigments concentrations, macroelements, and organic anions (malate, acetate and aconitate) in roots, were also increased. Desorption experiments, XPS analysis of the outer thin root surface (≤100Å) and µXRF analyses showed that Si increased adsorption of Cu at the root surface as well as Cu accumulation in the epidermis while Cu was localised in the central cylinder when Si was not applied. Copper was not detected in phytoliths. This study provides evidences for Si-mediated alleviation of Cu toxicity in durum wheat. It also shows that Si supplementation to plants exposed to increasing levels of Cu in solution induces non-simultaneous changes in physiological parameters. We propose a three-step mechanism occurring mainly at the root level and limiting Cu uptake and translocation to shoots: (i) increased Cu adsorption onto the outer thin layer root surface and immobilisation in the vicinity of root epidermis, (ii) increased Cu complexation by both inorganic and organic anions such as aconitate and, (iii) limitation of translocation through an enhanced thickening of a Si-loaded endodermis. Springer-Verlag Berlin Heidelberg, 2014 Anions nationallicence Copper nationallicence Durum wheat nationallicence Hydroponics nationallicence Element localisation nationallicence µXRF nationallicence Roots nationallicence Silicon nationallicence Keller C. CEREGE, UMR AMU-CNRS 7330, Aix-Marseille Univ., Technopôle de l'Environnement Arbois-Méditerranée, 13545, Aix en Provence Cedex 4, France aut Rizwan M. CEREGE, UMR AMU-CNRS 7330, Aix-Marseille Univ., Technopôle de l'Environnement Arbois-Méditerranée, 13545, Aix en Provence Cedex 4, France aut Davidian J.-C Biochimie and Physiologie Moléculaire des Plantes, UMR CNRS-INRA-Université Montpellier II-Montpellier SupAgro, Place Viala, 34060, Montpellier Cedex 2, France aut Pokrovsky O. UMR 5563 CNRS, Geoscience and Environment Toulouse (GET), 14 Avenue Edouard Belin, 31400, Toulouse, France aut Bovet N. NanoGeoScience, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark aut Chaurand P. CEREGE, UMR AMU-CNRS 7330, Aix-Marseille Univ., Technopôle de l'Environnement Arbois-Méditerranée, 13545, Aix en Provence Cedex 4, France aut Meunier J.-D CEREGE, UMR AMU-CNRS 7330, Aix-Marseille Univ., Technopôle de l'Environnement Arbois-Méditerranée, 13545, Aix en Provence Cedex 4, France aut Planta Springer Berlin Heidelberg 241/4(2015-04-01), 847-860 0032-0935 241:4<847 2015 241 425 https://doi.org/10.1007/s00425-014-2220-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2220-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keller C. CEREGE, UMR AMU-CNRS 7330, Aix-Marseille Univ., Technopôle de l'Environnement Arbois-Méditerranée, 13545, Aix en Provence Cedex 4, France aut NATIONALLICENCE 700 1- Rizwan M. CEREGE, UMR AMU-CNRS 7330, Aix-Marseille Univ., Technopôle de l'Environnement Arbois-Méditerranée, 13545, Aix en Provence Cedex 4, France aut NATIONALLICENCE 700 1- Davidian J.-C Biochimie and Physiologie Moléculaire des Plantes, UMR CNRS-INRA-Université Montpellier II-Montpellier SupAgro, Place Viala, 34060, Montpellier Cedex 2, France aut NATIONALLICENCE 700 1- Pokrovsky O. UMR 5563 CNRS, Geoscience and Environment Toulouse (GET), 14 Avenue Edouard Belin, 31400, Toulouse, France aut NATIONALLICENCE 700 1- Bovet N. NanoGeoScience, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Chaurand P. CEREGE, UMR AMU-CNRS 7330, Aix-Marseille Univ., Technopôle de l'Environnement Arbois-Méditerranée, 13545, Aix en Provence Cedex 4, France aut NATIONALLICENCE 700 1- Meunier J.-D CEREGE, UMR AMU-CNRS 7330, Aix-Marseille Univ., Technopôle de l'Environnement Arbois-Méditerranée, 13545, Aix en Provence Cedex 4, France aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/4(2015-04-01), 847-860 0032-0935 241:4<847 2015 241 425