caa a22 4500 606209212 CHVBK 20210128101027.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00425-014-2223-y doi (NATIONALLICENCE)springer-10.1007/s00425-014-2223-y Trichome differentiation on leaf primordia of Helianthus annuus (Asteraceae): morphology, gene expression and metabolite profile [Elektronische Daten] [Anna-Katharina Aschenbrenner, Evelyn Amrehn, Lisa Bechtel, Otmar Spring] Main conclusion : Sunflower trichomes fully develop on embryonic plumula within 3days after start of germination. Toxic sesquiterpene lactones are produced immediately thereafter thus protecting the apical bud of the seedling against herbivory. Helianthus annuus harbors non-glandular and two different types of multicellular glandular trichomes, namely the biseriate capitate glandular trichomes and the uniseriate linear glandular trichomes. The development of capitate glandular trichomes is well known from anther tips on sunflower disk florets, but not from leaves and no information is yet available on the development of the linear glandular trichomes. Scanning electron microscopy of sunflower seedlings unravelled that within the first 40h of seed germination all three types of trichomes started to emerge on primordia of the first true leaves. Within the following 20-30h trichomes developed from trichoblasts to fully differentiated hairs. Gene expression studies showed that genes involved in the trichome-based sesquiterpene lactone formation were up-regulated between 72 and 96h after start of germination. Metabolite profiling with HPLC confirmed the synthesis of sesquiterpene lactones which may contribute to protect the germinating seedlings from herbivory. The study has shown that sunflower leaf primordia can serve as a fast and easy to handle model system for the investigation of trichome development in Asteraceae. Springer-Verlag Berlin Heidelberg, 2014 Gene expression nationallicence Leaf primordia nationallicence Metabolite profiling nationallicence Scanning electron microscopy nationallicence Sesquiterpene lactones nationallicence Trichome differentiation nationallicence CGT : Capitate glandular trichomes nationallicence GT : Glandular trichomes nationallicence LGT : Linear glandular trichomes nationallicence NGT : Non-glandular trichomes nationallicence RT : Room temperature nationallicence SEM : Scanning electron microscopy nationallicence Aschenbrenner Anna-Katharina Institute of Botany, University of Hohenheim, Garbenstraße 30, 70593, Stuttgart, Germany aut Amrehn Evelyn Institute of Botany, University of Hohenheim, Garbenstraße 30, 70593, Stuttgart, Germany aut Bechtel Lisa Institute of Botany, University of Hohenheim, Garbenstraße 30, 70593, Stuttgart, Germany aut Spring Otmar Institute of Botany, University of Hohenheim, Garbenstraße 30, 70593, Stuttgart, Germany aut Planta Springer Berlin Heidelberg 241/4(2015-04-01), 837-846 0032-0935 241:4<837 2015 241 425 https://doi.org/10.1007/s00425-014-2223-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2223-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aschenbrenner Anna-Katharina Institute of Botany, University of Hohenheim, Garbenstraße 30, 70593, Stuttgart, Germany aut NATIONALLICENCE 700 1- Amrehn Evelyn Institute of Botany, University of Hohenheim, Garbenstraße 30, 70593, Stuttgart, Germany aut NATIONALLICENCE 700 1- Bechtel Lisa Institute of Botany, University of Hohenheim, Garbenstraße 30, 70593, Stuttgart, Germany aut NATIONALLICENCE 700 1- Spring Otmar Institute of Botany, University of Hohenheim, Garbenstraße 30, 70593, Stuttgart, Germany aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/4(2015-04-01), 837-846 0032-0935 241:4<837 2015 241 425