caa a22 4500 606209239 CHVBK 20210128101027.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00425-014-2218-8 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2218-8 Induction of plant virus defense response by brassinosteroids and brassinosteroid signaling in Arabidopsis thaliana [Elektronische Daten] [Da-Wei Zhang, Xing-Guang Deng, Fa-Qiong Fu, Hong-Hui Lin] Main conclusion : Our study demonstrated that CMV resistance was upregulated by brassinosteroids (BRs) treatment, and BR signaling was needed for this BRs-induced CMV tolerance. Plant steroid hormones, brassinosteroids (BRs), play essential roles in variety of plant developmental processes and adaptation to various biotic and abiotic stresses. BR signal through plasma membrane-localized receptor and other components to modulate several transcription factors that modulate thousands of target genes including certain stress-responsive genes. To study the effects of BRs on plant virus defense and how BRs induce plant virus stress tolerance, we manipulated the BRs levels in Arabidopsis thaliana and found that BRs levels were positively correlated with the tolerance to Cucumber mosaic virus (CMV). We also showed that BRs treatment alleviated photosystem damage, enhanced antioxidant enzymes activity and induced defense-associated genes expression under CMV stress in Arabidopsis. To see whether BR signaling is essential for the plant virus defense response, we made use of BR signaling mutants (a weak allele of the BRs receptor mutant bri1-5 and constitutive BRs response mutant bes1-D). Compared with wild-type Arabidopsis plants, bri1-5 displayed reversed tolerance to CMV, but the resistance was enhanced in bes1-D. Together our results suggest that BRs can induce plant virus defense response through BR signaling. Springer-Verlag Berlin Heidelberg, 2014 Arabidopsis nationallicence BES1 nationallicence Brassinosteroid nationallicence BRI1 nationallicence CMV nationallicence Defense nationallicence BR : Brassinosteroid nationallicence BL : Brassinolide nationallicence BRZ : Brassinazole nationallicence BRI1 : BRASSINOSTEROID-INSENSITIVE1 nationallicence BES1 : BRI1 EMS SUPRESSOR 1 nationallicence CMV : Cucumber mosaic virus nationallicence ROS : Reactive oxygen species nationallicence Dpi : Days postinoculation nationallicence Zhang Da-Wei Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, 610064, Chengdu, Sichuan, China aut Deng Xing-Guang Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, 610064, Chengdu, Sichuan, China aut Fu Fa-Qiong Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, 610064, Chengdu, Sichuan, China aut Lin Hong-Hui Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, 610064, Chengdu, Sichuan, China aut Planta Springer Berlin Heidelberg 241/4(2015-04-01), 875-885 0032-0935 241:4<875 2015 241 425 https://doi.org/10.1007/s00425-014-2218-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2218-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Da-Wei Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, 610064, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Deng Xing-Guang Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, 610064, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Fu Fa-Qiong Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, 610064, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Lin Hong-Hui Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, 610064, Chengdu, Sichuan, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/4(2015-04-01), 875-885 0032-0935 241:4<875 2015 241 425