caa a22 4500 606209255 CHVBK 20210128101027.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00425-014-2221-0 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2221-0 Redox proteomics and physiological responses in Cistus albidus shrubs subjected to long-term summer drought followed by recovery [Elektronische Daten] [Ricard Brossa, Marta Pintó-Marijuan, Rita Francisco, Marta López-Carbonell, Maria Chaves, Leonor Alegre] Main conclusion : The interaction between enzymatic and non-enzymatic antioxidants, endogenous levels of ABA and ABA-GE, the rapid recuperation of photosynthetic proteins under re-watering as well the high level of antioxidant proteins in previously drought-stressed plants under re-watering conditions, will contribute to drought resistance in plants subjected to a long-term drought stress under Mediterranean field conditions. This work provides an overview of the mechanisms of Cistus albidus acclimation to long-term summer drought followed by re-watering in Mediterranean field conditions. To better understand the molecular mechanisms of drought resistance in these plants, a proteomic study using 2-DE and MALDI-TOF/TOF MS/MS was performed on leaves from these shrubs. The analysis identified 57 differentially expressed proteins in water-stressed plants when contrasted to well watered. Water-stressed plants showed an increase, both qualitatively and quantitatively, in HSPs, and downregulation of photosynthesis and carbon metabolism enzymes. Under drought conditions, there was considerable upregulation of enzymes related to redox homeostasis, DHA reductase, Glyoxalase, SOD and isoflavone reductase. However, upregulation of catalase was not observed until after re-watering was carried out. Drought treatment caused an enhancement in antioxidant defense responses that can be modulated by ABA, and its catabolites, ABA-GE, as well as JA. Furthermore, quantification of protein carbonylation was shown to be a useful marker of the relationship between water and oxidative stress, and showed that there was only moderate oxidative stress in C. albidus plants subjected to water stress. After re-watering plants recovered although the levels of ABA-GE and antioxidant enzymes still remain higher than in well-watered plants. We expect that our results will provide new data on summer acclimation to drought stress in Mediterranean shrubs. The Author(s), 2014 Antioxidants nationallicence Photosynthesis nationallicence Plant hormones nationallicence Protein carbonylation nationallicence Water stress nationallicence ABA : Abscisic acid nationallicence ABA-GE : β-D glucosyl ester nationallicence APX : Ascorbate peroxidase nationallicence AsA : Ascorbate nationallicence CAT : Catalase nationallicence DHA : Oxidized ascorbate nationallicence DPA : Dehydrophaseic nationallicence GSH : Reduced glutathione nationallicence GSSFG : Oxidized glutathione nationallicence JA : Jasmonic acid nationallicence PA : Phaseic acid nationallicence ROS : Reactive oxygen species nationallicence SOD : Superoxide dismutase nationallicence WS : Water stressed nationallicence WW : Well watered nationallicence Brossa Ricard Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut Pintó-Marijuan Marta Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut Francisco Rita Molecular Ecophysiology Lab. (LEM), Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780-901, Oeiras, Portugal aut López-Carbonell Marta Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut Chaves Maria Molecular Ecophysiology Lab. (LEM), Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780-901, Oeiras, Portugal aut Alegre Leonor Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut Planta Springer Berlin Heidelberg 241/4(2015-04-01), 803-822 0032-0935 241:4<803 2015 241 425 https://doi.org/10.1007/s00425-014-2221-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2221-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brossa Ricard Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Pintó-Marijuan Marta Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Francisco Rita Molecular Ecophysiology Lab. (LEM), Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780-901, Oeiras, Portugal aut NATIONALLICENCE 700 1- López-Carbonell Marta Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Chaves Maria Molecular Ecophysiology Lab. (LEM), Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780-901, Oeiras, Portugal aut NATIONALLICENCE 700 1- Alegre Leonor Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/4(2015-04-01), 803-822 0032-0935 241:4<803 2015 241 425