caa a22 4500 606209271 CHVBK 20210128101027.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00425-014-2226-8 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2226-8 Identification, validation and cross-species transferability of novel Lavandula EST-SSRs [Elektronische Daten] [Ayelign Adal, Zerihun Demissie, Soheil Mahmoud] Main conclusion : We identified and characterized EST-SSRs with strong discrimination power against Lavandula angustifolia and Lavandula x intermedia . The markers also showed considerable cross-species transferability rate into six related Lavandula species. Abstract: Lavenders (Lavandula) are important economical crops grown around the globe for essential oil production. In an attempt to develop genetic markers for these plants, we analyzed over 13,000 unigenes developed from L. angustifolia and L. x intermedia EST databases, and identified 3,459 simple sequence repeats (SSR), which were dominated by trinucleotides (41.2%) and dinucleotides (31.45%). Approximately, 19% of the unigenes contained at least one SSR marker, over 60% of which were localized in the UTRs. Only 252 EST-SSRs were 18bp or longer from which 31 loci were validated, and 24 amplified discrete fragments with 85% polymorphism in L. x intermedia and L. angustifolia. The average number of alleles in L. x intermedia and L. angustifolia were 3.42 and 3.71 per marker with average PIC values of 0.47 and 0.52, respectively. These values suggest a moderate to strong level of informativeness for the markers, with some loci producing unique fingerprints. The cross-species transferability rate of the markers ranges 50-100% across eight species. The utility of these markers was assessed in eight Lavandula species and 15 L. angustifolia and L. x intermedia cultivars, and the dendrogram deduced from their similarity indexes successfully delineated the species into their respective sections and the cultivars into their respective species. These markers have potential for application in fingerprinting, diversity studies and marker-assisted breeding of Lavandula. Springer-Verlag Berlin Heidelberg, 2014 EST-SSR nationallicence Genetic marker nationallicence Lavandula nationallicence L. angustifolia nationallicence L. x intermedia nationallicence Polymorphism nationallicence CDS : Protein coding regions nationallicence EST : Expressed sequence tags nationallicence EO : Essential oil nationallicence He : Genetic diversity nationallicence Na : Number of alleles nationallicence PIC : Polymorphic information content nationallicence PFR : Primer flanking regions nationallicence SSR : Simple sequence repeats nationallicence UTR : Untranslated regions nationallicence Adal Ayelign Department of Biology, University of British Columbia, 1177 Research Rd, V1V 1V7, Kelowna, BC, Canada aut Demissie Zerihun Department of Biology, University of British Columbia, 1177 Research Rd, V1V 1V7, Kelowna, BC, Canada aut Mahmoud Soheil Department of Biology, University of British Columbia, 1177 Research Rd, V1V 1V7, Kelowna, BC, Canada aut Planta Springer Berlin Heidelberg 241/4(2015-04-01), 987-1004 0032-0935 241:4<987 2015 241 425 https://doi.org/10.1007/s00425-014-2226-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2226-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Adal Ayelign Department of Biology, University of British Columbia, 1177 Research Rd, V1V 1V7, Kelowna, BC, Canada aut NATIONALLICENCE 700 1- Demissie Zerihun Department of Biology, University of British Columbia, 1177 Research Rd, V1V 1V7, Kelowna, BC, Canada aut NATIONALLICENCE 700 1- Mahmoud Soheil Department of Biology, University of British Columbia, 1177 Research Rd, V1V 1V7, Kelowna, BC, Canada aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/4(2015-04-01), 987-1004 0032-0935 241:4<987 2015 241 425