caa a22 4500 60620928X CHVBK 20210128101027.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00425-014-2209-9 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2209-9 Comparative proteomic analysis reveals the role of hydrogen sulfide in the adaptation of the alpine plant Lamiophlomis rotata to altitude gradient in the Northern Tibetan Plateau [Elektronische Daten] [Lan Ma, Liming Yang, Jingjie Zhao, Jingjing Wei, Xiangxiang Kong, Chuntao Wang, Xiaoming Zhang, Yongping Yang, Xiangyang Hu] Main conclusion : We found the novel role of hydrogen sulfide in the adaptation of the alpine plant to altitude gradient in the Northern Tibetan Plateau. Alpine plants have developed strategies to survive the extremely cold conditions prevailing at high altitudes; however, the mechanism underlying the evolution of these strategies remains unknown. Hydrogen sulfide (H2S) is an essential messenger that enhances plant tolerance to environmental stress; however, its role in alpine plant adaptation to environmental stress has not been reported until now. In this work, we conducted a comparative proteomics analysis to investigate the dynamic patterns of protein expression in Lamiophlomis rotata plants grown at three different altitudes. We identified and annotated 83 differentially expressed proteins. We found that the levels and enzyme activities of proteins involved in H2S biosynthesis markedly increased at higher altitudes, and that H2S accumulation increased. Exogenous H2S application increased antioxidant enzyme activity, which reduced ROS (reactive oxygen species) damage, and GSNOR (S-nitrosoglutathione reductase) activity, which reduced RNS (reactive nitrogen species) damage, and activated the downstream defense response, resulting in protein degradation and proline and sugar accumulation. However, such defense responses could be reversed by applying H2S biosynthesis inhibitors. Based on these findings, we conclude that L. rotata uses multiple strategies to adapt to the alpine stress environment and that H2S plays a central role during this process. Springer-Verlag Berlin Heidelberg, 2014 Adaptation nationallicence H2S nationallicence Lamiophlomis nationallicence Proteomics nationallicence APX : Ascorbate peroxidase nationallicence CAS : Beta-Cyanoalanine synthase nationallicence CAT : Catalase nationallicence CBS : Cystathionine b-synthase nationallicence CO : Carbon monoxide nationallicence CSC : Cysteine synthesis complex nationallicence CSE : Cystathionine c lyase nationallicence D-CD : d-Cysteine desulfhydrase nationallicence FDR : False discovery rates nationallicence GSH : Glutathione nationallicence GSNOR : S-nitrosoglutathione reductase nationallicence H2S : Hydrogen sulfide nationallicence D/L-CDs : d/l-cysteine desulfhydrases nationallicence NO : Nitric oxide nationallicence OAS : O-acetyl serine nationallicence OAS-TL : O-acetyl-thiol-serinelyase nationallicence ROS : Reactive oxygen species nationallicence RNS : Reactive nitrogen species nationallicence SOD : Superoxide dismutase nationallicence Ma Lan Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut Yang Liming School of Life Sciences, Jiangsu Key Laboratory for Eco-Agriculture Biotechnology Around Hongze Lake, Huayin Normal University, 223300, Huai'an, China aut Zhao Jingjie Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut Wei Jingjing Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut Kong Xiangxiang Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut Wang Chuntao Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut Zhang Xiaoming Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut Yang Yongping Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut Hu Xiangyang Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut Planta Springer Berlin Heidelberg 241/4(2015-04-01), 887-906 0032-0935 241:4<887 2015 241 425 https://doi.org/10.1007/s00425-014-2209-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2209-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Lan Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Yang Liming School of Life Sciences, Jiangsu Key Laboratory for Eco-Agriculture Biotechnology Around Hongze Lake, Huayin Normal University, 223300, Huai'an, China aut NATIONALLICENCE 700 1- Zhao Jingjie Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Wei Jingjing Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Kong Xiangxiang Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Wang Chuntao Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Zhang Xiaoming Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Yang Yongping Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut NATIONALLICENCE 700 1- Hu Xiangyang Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, 650201, Kunming, Yunnan, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/4(2015-04-01), 887-906 0032-0935 241:4<887 2015 241 425