caa a22 4500 606209301 CHVBK 20210128101027.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00425-014-2222-z doi (NATIONALLICENCE)springer-10.1007/s00425-014-2222-z Isolation and characterization of an ubiquitin extension protein gene ( JcUEP ) promoter from Jatropha curcas [Elektronische Daten] [Yan-Bin Tao, Liang-Liang He, Long-Jian Niu, Zeng-Fu Xu] Main conclusion : The JcUEP promoter is active constitutively in the bio-fuel plant Jatropha curcas , and is an alternative to the widely used CaMV35S promoter for driving constitutive overexpression of transgenes in Jatropha. Well-characterized promoters are required for transgenic breeding of Jatropha curcas, a biofuel feedstock with great potential for production of bio-diesel and bio-jet fuel. In this study, an ubiquitin extension protein gene from Jatropha, designated JcUEP, was identified to be ubiquitously expressed. Thus, we isolated a 1.2kb fragment of the 5′ flanking region of JcUEP and evaluated its activity as a constitutive promoter in Arabidopsis and Jatropha using the β-glucuronidase (GUS) reporter gene. As expected, histochemical GUS assay showed that the JcUEP promoter was active in all Arabidopsis and Jatropha tissues tested. We also compared the activity of the JcUEP promoter with that of the cauliflower mosaic virus 35S (CaMV35S) promoter, a well-characterized constitutive promoter conferring strong transgene expression in dicot species, in various tissues of Jatropha. In a fluorometric GUS assay, the two promoters showed similar activities in stems, mature leaves and female flowers; while the CaMV35S promoter was more effective than the JcUEP promoter in other tissues, especially young leaves and inflorescences. In addition, the JcUEP promoter retained its activity under stress conditions in low temperature, high salt, dehydration and exogenous ABA treatments. These results suggest that the plant-derived JcUEP promoter could be an alternative to the CaMV35S promoter for driving constitutive overexpression of transgenes in Jatropha and other plants. Springer-Verlag Berlin Heidelberg, 2014 Physic nut nationallicence Constitutive promoter nationallicence Ubiquitin nationallicence Transgenic nationallicence Stress nationallicence CaMV35S nationallicence Tao Yan-Bin Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Menglun, Yunnan, China aut He Liang-Liang Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Menglun, Yunnan, China aut Niu Long-Jian Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Menglun, Yunnan, China aut Xu Zeng-Fu Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Menglun, Yunnan, China aut Planta Springer Berlin Heidelberg 241/4(2015-04-01), 823-836 0032-0935 241:4<823 2015 241 425 https://doi.org/10.1007/s00425-014-2222-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2222-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tao Yan-Bin Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Menglun, Yunnan, China aut NATIONALLICENCE 700 1- He Liang-Liang Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Menglun, Yunnan, China aut NATIONALLICENCE 700 1- Niu Long-Jian Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Menglun, Yunnan, China aut NATIONALLICENCE 700 1- Xu Zeng-Fu Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Menglun, Yunnan, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/4(2015-04-01), 823-836 0032-0935 241:4<823 2015 241 425