caa a22 4500 606209387 CHVBK 20210128101028.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00425-015-2249-9 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2249-9 Sulphur responsiveness of the Chlamydomonas reinhardtii LHCBM9 promoter [Elektronische Daten] [Anne Sawyer, Ben Hankamer, Ian Ross] Main conclusion : A 44-base-pair region in the Chlamydomonas reinhardtii LHCBM9 promoter is essential for sulphur responsiveness. The photosynthetic light-harvesting complex (LHC) proteins play essential roles both in light capture, the first step of photosynthesis, and in photoprotective mechanisms. In contrast to the other LHC proteins and the majority of photosynthesis proteins, the Chlamydomonas reinhardtii photosystem II-associated LHC protein, LHCBM9, was recently reported to be up-regulated under sulphur deprivation conditions, which also induce hydrogen production. Here, we examined the sulphur responsiveness of the LHCBM9 gene at the transcriptional level, through promoter deletion analysis. The LHCBM9 promoter was found to be responsive to sulphur deprivation, with a 44-base-pair region between nucleotide positions −136 and −180 relative to the translation start site identified as essential for this response. Anaerobiosis was found to enhance promoter activity under sulphur deprivation conditions, however, alone was unable to induce promoter activity. The study of LHCBM9 is of biological and biotechnological importance, as its expression is linked to photobiological hydrogen production, theoretically the most efficient process for biofuel production, while the simplicity of using an S-deprivation trigger enables the development of a novel C. reinhardtii-inducible promoter system based on LHCBM9. Springer-Verlag Berlin Heidelberg, 2015 Algae nationallicence Light harvesting nationallicence Deletion analysis nationallicence Luciferase nationallicence Sawyer Anne Institute for Molecular Bioscience, The University of Queensland, 4072, Brisbane, QLD, Australia aut Hankamer Ben Institute for Molecular Bioscience, The University of Queensland, 4072, Brisbane, QLD, Australia aut Ross Ian Institute for Molecular Bioscience, The University of Queensland, 4072, Brisbane, QLD, Australia aut Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1287-1302 0032-0935 241:5<1287 2015 241 425 https://doi.org/10.1007/s00425-015-2249-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2249-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sawyer Anne Institute for Molecular Bioscience, The University of Queensland, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Hankamer Ben Institute for Molecular Bioscience, The University of Queensland, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Ross Ian Institute for Molecular Bioscience, The University of Queensland, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1287-1302 0032-0935 241:5<1287 2015 241 425