caa a22 4500 606209395 CHVBK 20210128101028.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00425-015-2253-0 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2253-0 Molecular integrity of chloroplast DNA and mitochondrial DNA in mesophyll and bundle sheath cells of maize [Elektronische Daten] [Rachana Kumar, Delene Oldenburg, Arnold Bendich] Main conclusion : When compared to maize mesophyll cells, the plastid and mitochondrial DNAs in bundle sheath cells are less fragmented, less damaged, and contain fewer DNA polymerase-blocking impediments. Plants that conduct C4 photosynthesis differ from those that employ C3 photosynthesis with respect to leaf anatomy, biochemical pathways, and the proteins and RNA transcripts present in the leaf mesophyll (M) and bundle sheath (BS) cells. Here, we investigate the organellar DNA (orgDNA) from plastids and mitochondria in these two cell types. We use standard qPCR, long PCR, and DNA damage analysis to quantify the amount and quality of orgDNA in isolated M and BS cells of maize. When compared to M cells, BS cells have less orgDNA damage and a higher percentage of unimpeded orgDNA. In addition, the orgDNA is more fragmented in M than BS cells, although orgDNA in BS is subject to more in vitro repair. We suggest that the differences in molecular integrity of orgDNA in these two cells are due to higher levels of reactive oxygen species in M than BS cells. Springer-Verlag Berlin Heidelberg, 2015 Bundle sheath nationallicence DNA damage nationallicence DNA repair nationallicence Mesophyll nationallicence Organellar DNA nationallicence Reactive oxygen species nationallicence Zea mays nationallicence BS : Bundle sheath nationallicence M : Mesophyll nationallicence miPCR : Molecular integrity PCR nationallicence mtDNA : Mitochondrial DNA nationallicence nucDNA : Nuclear DNA nationallicence orgDNA : Organellar DNA nationallicence ptDNA : Plastid DNA nationallicence qPCR : Real-time quantitative PCR nationallicence ROS : Reactive oxygen species nationallicence ttDNA : Total tissue DNA nationallicence Kumar Rachana Department of Biology, University of Washington, 98195-5325, Seattle, WA, USA aut Oldenburg Delene Department of Biology, University of Washington, 98195-5325, Seattle, WA, USA aut Bendich Arnold Department of Biology, University of Washington, 98195-5325, Seattle, WA, USA aut Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1221-1230 0032-0935 241:5<1221 2015 241 425 https://doi.org/10.1007/s00425-015-2253-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2253-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumar Rachana Department of Biology, University of Washington, 98195-5325, Seattle, WA, USA aut NATIONALLICENCE 700 1- Oldenburg Delene Department of Biology, University of Washington, 98195-5325, Seattle, WA, USA aut NATIONALLICENCE 700 1- Bendich Arnold Department of Biology, University of Washington, 98195-5325, Seattle, WA, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1221-1230 0032-0935 241:5<1221 2015 241 425