caa a22 4500 606209417 CHVBK 20210128101028.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00425-015-2245-0 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2245-0 Abscisic acid and the key enzymes and genes in sucrose-to-starch conversion in rice spikelets in response to soil drying during grain filling [Elektronische Daten] [Zhiqin Wang, Yunji Xu, Tingting Chen, Hao Zhang, Jianchang Yang, Jianhua Zhang] Main conclusion : Abscisic acid mediates the effect of post-anthesis soil drying on grain filling through regulating the activities of key enzymes and expressions of genes involved in sucrose-to-starch conversion in rice spikelets. This study investigated if abscisic acid (ABA) would mediate the effect of post-anthesis soil drying on grain filling through regulating the key enzymes in sucrose-to-starch conversion in rice (Oryza sativa L.) spikelets. Two rice cultivars were field-grown. Three treatments, well-watered (WW), moderate soil drying (MD), and severe soil drying (SD), were imposed from 6days after full heading until maturity. When compared with those under the WW, grain filling rate, grain weight, and sink activity, in terms of the activities and gene expression levels of sucrose synthase, ADP glucose pyrophosphorylase, starch synthase, and starch branching enzyme, in inferior spikelets were substantially increased under the MD, whereas they were markedly decreased in both superior and inferior spikelets under the SD. The two cultivars showed the same tendencies. Both MD and SD increased ABA content and expression levels of its biosynthesis genes in spikelets, with more increase under the SD than the MD. ABA content was significantly correlated with grain filling rate and sink activities under both WW and MD, while the correlations were not significant under the SD. Application of a low concentration ABA to WW plants imitated the results under the MD, and applying with a high concentration ABA showed the effect of the SD. The results suggest that ABA plays a vital role in grain filling through regulating sink activity and functions in a dose-dependent manner. An elevated ABA level under the MD enhances, whereas a too high level of ABA under the SD decreases, sink activity. Springer-Verlag Berlin Heidelberg, 2015 Inferior spikelets nationallicence Oryza (rice) nationallicence Sink activity nationallicence Starch biosynthesis nationallicence Superior spikelets nationallicence ABA : Abscisic acid nationallicence AGPase : ADP glucose pyrophosphorylase nationallicence AGPL : ADP glucose pyrophosphorylase large subunit nationallicence AGPS : ADP glucose pyrophosphorylase small subunit nationallicence AI : Acid invertase nationallicence CIN : Cell-wall invertase nationallicence DAH : Days after heading nationallicence DPA : Days post-anthesis nationallicence GBSS : Granule-bound starch synthase nationallicence INV : Vacuolar invertase nationallicence MD : Moderate soil drying nationallicence NCED : 9-Cis-epoxycarotenoid dioxygenase nationallicence ψsoil : Soil water potential nationallicence SBE : Starch branching enzyme nationallicence SD : Severe soil drying nationallicence SSS : Soluble starch synthase nationallicence StSase : Starch synthase nationallicence SuSase : Sucrose synthase nationallicence WW : Well-watered nationallicence Wang Zhiqin Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China aut Xu Yunji Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China aut Chen Tingting Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China aut Zhang Hao Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China aut Yang Jianchang Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China aut Zhang Jianhua School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China aut Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1091-1107 0032-0935 241:5<1091 2015 241 425 https://doi.org/10.1007/s00425-015-2245-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2245-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Zhiqin Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 700 1- Xu Yunji Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 700 1- Chen Tingting Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 700 1- Zhang Hao Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 700 1- Yang Jianchang Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China aut NATIONALLICENCE 700 1- Zhang Jianhua School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1091-1107 0032-0935 241:5<1091 2015 241 425