caa a22 4500 606209425 CHVBK 20210128101028.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00425-015-2242-3 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2242-3 Rice RING E3 ligase may negatively regulate gamma-ray response to mediate the degradation of photosynthesis-related proteins [Elektronische Daten] [Yong Park, Jung Kim, Dong Kim, Cheol Jang] Main conclusion : In this study, our findings regarding the regulation of GA irradiation-induced OsGIRP1 in relation to the levels of photosynthesis-related proteins such as OsrbcL1 and OsrbcS1 and hypersensitive responses of overexpressing plants to GR irradiation provide insight into the molecular functions of OsGIRP1 as a negative regulator in response to the stress of radiation. Abstract: The RING (Really Interesting New Gene) finger proteins are known to play crucial roles in various abiotic stresses in plants. Here, we report on RING finger E3 ligase, Oryza sativa gamma rays-induced RING finger protein1 gene (OsGIRP1), which is highly induced by gamma rays (GR) irradiation. In vitro ubiquitination assay demonstrated that a single amino acid substitution (OsGIRP1C196A) of the RING domain showed no E3 ligase activity, supporting the notion that the activity of most E3s is specified by a RING domain. We isolated at least 6 substrate proteins of OsGIRP1, including 2 Rubisco subunits, OsrbcL1 and OsrbcSl, via yeast two-hybridization and bimolecular fluorescence complementation assays. OsGIRP1 and its partner proteins were targeted to the cytosol and the cytosol or chloroplasts, respectively; however, florescence signals of the complexes with OsGIPR1 were observed in the cytosol. Protein degradation in cell extracts showed that OsGIRP1 mediates proteolysis of 2 substrates, OsrbcS1 and OsrbcL1, via the 26S proteasome degradation pathway. The Arabidopsis plants overexpressing OsGIRP1 clearly exhibited increased sensitivity to GR irradiation. These results might suggest that OsGIRP1 acts as a negative regulator of GR response to mediate the degradation of photosynthesis-related proteins. Springer-Verlag Berlin Heidelberg, 2015 26S proteasome nationallicence Gamma rays nationallicence RING E3 ligase nationallicence Rubisco subunits nationallicence Ubiquitination nationallicence RING : Really interesting new gene nationallicence GR : Gamma rays nationallicence BiFC : Bimolecular fluorescence complementation nationallicence Rubisco : Ribulose biphosphate carboxylase/oxygenase nationallicence DDO/X/A : Synthetic defined medium lacking Leu and Trp supplemented with 40μgml−1 X-α-Gal and 70ngml−1 aureobasidin A (AbA) nationallicence QDO/X/A : Synthetic defined medium lacking Ade, His, Leu, and Trp with 40μgml−1 X-α-Gal and 70ngml−1 AbA nationallicence Park Yong Plant Genomics Lab, Department of Applied Plant Sciences, Kangwon National University, 200-713, Chuncheon, Republic of Korea aut Kim Jung Plant Genomics Lab, Department of Applied Plant Sciences, Kangwon National University, 200-713, Chuncheon, Republic of Korea aut Kim Dong Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong, 580-185, Jeongeup, Jeonbuk, South Korea aut Jang Cheol Plant Genomics Lab, Department of Applied Plant Sciences, Kangwon National University, 200-713, Chuncheon, Republic of Korea aut Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1119-1129 0032-0935 241:5<1119 2015 241 425 https://doi.org/10.1007/s00425-015-2242-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2242-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Yong Plant Genomics Lab, Department of Applied Plant Sciences, Kangwon National University, 200-713, Chuncheon, Republic of Korea aut NATIONALLICENCE 700 1- Kim Jung Plant Genomics Lab, Department of Applied Plant Sciences, Kangwon National University, 200-713, Chuncheon, Republic of Korea aut NATIONALLICENCE 700 1- Kim Dong Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong, 580-185, Jeongeup, Jeonbuk, South Korea aut NATIONALLICENCE 700 1- Jang Cheol Plant Genomics Lab, Department of Applied Plant Sciences, Kangwon National University, 200-713, Chuncheon, Republic of Korea aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1119-1129 0032-0935 241:5<1119 2015 241 425