caa a22 4500 606209433 CHVBK 20210128101028.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00425-015-2241-4 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2241-4 Sulfate influx transporters in Arabidopsis thaliana are not involved in arsenate uptake but critical for tissue nutrient status and arsenate tolerance [Elektronische Daten] [Manal El-Zohri, Victor Odjegba, Lena Ma, Bala Rathinasabapathi] Main conclusion : Arsenic, a non-nutrient metalloid is toxic to plants but many details on the physiology of plant adaptation to arsenic stress are not well understood. This work provides new insights about the role of sulfur assimilation in arsenate uptake, growth and arsenic tolerance. Research reported here indicates that two high affinity sulfate transporters in Arabidopsis thaliana are not involved in root uptake of arsenate. Further this study revealed that sulfate status influenced thiol levels, elemental nutrients, growth and arsenate tolerance. The hypothesis that arsenate may be transported via sulfate transporters, SULTR1;1 and SULTR1;2 in Arabidopsis, was tested. The double mutant of sultr1;1 sultr1;2 exhibited significantly less growth than the wild-type or the single mutants. The double mutant's sulfur content was significantly lower than the wild-type but the single mutants were similar to the wild-type confirming the redundant functions of SULTR1;1 and SULTR1;2. Gene expression analyses indicated that the double mutant's sulfate uptake could be explained by the expressions of SULTR1;3, SULTR2;1, and SULTR2;2 in its roots. Following arsenate supply to the roots, the double mutant accumulated significantly less arsenic in the roots and the shoots than did the single mutants and the wild-type. The double mutant accumulated significantly less potassium and phosphorus also. 35S sulfate supplied to wild-type or double mutant roots showed that sulfate uptake was not inhibited by arsenate. Taken together, these results indicate that root uptake of arsenate is probably not via sulfate transporters, but the poor growth of the double mutant of sultr1;1 and sultr1;2 was due to its poor sulfate status and decreased levels of thiols, which had pleiotropic effects on the root uptake and translocation of potassium and phosphorus and arsenic tolerance. Springer-Verlag Berlin Heidelberg, 2015 Arabidopsis nationallicence Arsenic nationallicence Gene expression nationallicence Mutants nationallicence Phosphorus nationallicence Potassium nationallicence Sulfur nutrition nationallicence As(V) : Arsenate nationallicence As(III) : Arsenite nationallicence DTNB : 5,5' Dithiobis (2-nitrobenzoic acid) nationallicence ICP-AES : Inductively coupled plasma atomic emission spectrometry nationallicence ICP-MS : Inductively coupled plasma mass spectrometry nationallicence RT-PCR : Reverse transcription-polymerase chain reaction nationallicence SULTR : sulfate transporter nationallicence El-Zohri Manal Horticultural Sciences Department, University of Florida, 32611, Gainesville, FL, USA aut Odjegba Victor Horticultural Sciences Department, University of Florida, 32611, Gainesville, FL, USA aut Ma Lena Soil and Water Science Department, University of Florida, 32611, Gainesville, FL, USA aut Rathinasabapathi Bala Horticultural Sciences Department, University of Florida, 32611, Gainesville, FL, USA aut Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1109-1118 0032-0935 241:5<1109 2015 241 425 https://doi.org/10.1007/s00425-015-2241-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2241-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- El-Zohri Manal Horticultural Sciences Department, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Odjegba Victor Horticultural Sciences Department, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Ma Lena Soil and Water Science Department, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Rathinasabapathi Bala Horticultural Sciences Department, University of Florida, 32611, Gainesville, FL, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1109-1118 0032-0935 241:5<1109 2015 241 425