caa a22 4500 60620945X CHVBK 20210128101028.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00425-015-2259-7 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2259-7 Anther response to high-temperature stress during development and pollen thermotolerance heterosis as revealed by pollen tube growth and in vitro pollen vigor analysis in upland cotton [Elektronische Daten] [Guicheng Song, Miaomiao Wang, Bin Zeng, Jing Zhang, Chenliang Jiang, Qirui Hu, Guangtao Geng, Canming Tang] Main conclusion : Pollen tube growth in styles was strongly inhibited by temperature above 35°C, and the yield of cotton decreased because of the adverse effect of high temperatures during square development. High-temperature stress during flowering influences the square development of upland cotton (Gossypium hirsutum L.) and cotton yield. Although it is well known that square development is sensitive to high temperature, high-temperature sensitive stages of square development and the effects of high temperature on pollen tube growth in the styles are unknown. The effect of high temperature on anther development corresponding to pollen vigor is unknown during anther development. The objectives of this study were to identify the stages of square development that are sensitive to high temperatures (37/30 and 40/34°C), to determine whether the abnormal development of squares influenced by high temperature is responsible for the variation in the in vitro germination percent of pollen grains at anthesis, to identify the effect of high temperature on pollen germination in the styles, and to determine pollen thermotolerance heterosis. Our results show that the stages from the sporogenous cell to tetrad stage (square length <6.0mm) were the most sensitive to high temperature, and the corresponding pollen viability at anthesis was consistent with the changes in the square development stage. Pollen tube growth in the styles was strongly inhibited by temperature above 35°C, and the yield of cotton decreased because of the effect of high temperature during square development. The thermotolerance of hybrid F1 pollen showed heterosis, and pollen viability could be used as a criterion for screening for high-temperature tolerance cultivars. These results can be used in breeding to develop new cotton cultivars that can withstand high-temperature conditions, particularly in a future warmer climate. Springer-Verlag Berlin Heidelberg, 2015 Gossypium hirsutum L nationallicence High-temperature stress nationallicence Microspore nationallicence Pollen germination nationallicence Pollen mother cell nationallicence Tetrad nationallicence Ht : High temperature nationallicence HTS : High-temperature stress nationallicence Pg : Pollen germination nationallicence PGP : Pollen germination percentage nationallicence PMC : Pollen mother cell nationallicence PTL : Pollen tube length nationallicence PTn : Pollen tube number nationallicence SE : Standard error nationallicence Tukey's HSD : Tukey's honest significant difference nationallicence Song Guicheng State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Wang Miaomiao State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Zeng Bin State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Zhang Jing State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Jiang Chenliang State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Hu Qirui State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Geng Guangtao State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Tang Canming State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1271-1285 0032-0935 241:5<1271 2015 241 425 https://doi.org/10.1007/s00425-015-2259-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2259-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Guicheng State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Wang Miaomiao State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zeng Bin State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jing State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Chenliang State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Hu Qirui State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Geng Guangtao State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Tang Canming State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/5(2015-05-01), 1271-1285 0032-0935 241:5<1271 2015 241 425