caa a22 4500 606209522 CHVBK 20210128101029.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00425-014-2195-y doi (NATIONALLICENCE)springer-10.1007/s00425-014-2195-y Phenotyping carrot ( Daucus carota L.) for yield-determining temperature response by calorespirometry [Elektronische Daten] [Amaia Nogales, Luz Muñoz-Sanhueza, Lee Hansen, Birgit Arnholdt-Schmitt] Main conclusion : Calorespirometric measurements proved to be useful for phenotyping temperature response in terms of optimum temperatures for growth and low temperature limits for growth respiration in diverse carrot genotypes. High and low-temperature tolerance is an important trait in many breeding programs, but to date, improvement strategies have had limited success. Developing new, cost efficient and reliable screening tools to identify and select the most tolerant crop plant genotypes is necessary to assist plant breeding on cold and heat tolerance, and calorespirometry is proposed for this. Calorespirometry is a technique to simultaneously measure metabolic heat rates and CO2 emission rates of respiring tissues and can be used as a rapid method to determine how changes in the environment (e.g., temperature) influence plant growth. The main aim of this work was, therefore, to test the usefulness of calorespirometry as a phenotyping tool for carrot taproot growth in response to temperature. Calorespirometric measurements in the carrot taproot meristems of plants from eight carrot inbred lines allowed identification of optimum and minimum temperatures for growth of plants and to distinguish between phenotypes based on those characteristics. The technique proved to be useful for predicting yield-determining temperature responses in diverse carrot genotypes. Preliminary screening of new crop plant genotypes with calorespirometry based on their temperature adaptation and acclimation capability could make the screening process much less laborious by allowing selection of genotypes presenting the best growth performance under particular biotic or abiotic conditions before field tests. Springer-Verlag Berlin Heidelberg, 2014 Respiration nationallicence Growth rate nationallicence Cold nationallicence Optimum temperature nationallicence Low temperature limit for growth nationallicence Nogales Amaia EU Marie Curie Chair, ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, IIFA-Instituto de Formação e Investigação Avançada, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554, Évora, Portugal aut Muñoz-Sanhueza Luz Department of Plant Sciences (IPV), Norwegian University of Life Sciences (NMBU), P.O. Box 5003, 1432, Ås, Norway aut Hansen Lee Department of Chemistry and Biochemistry, Brigham Young University, 84602, Provo, UT, USA aut Arnholdt-Schmitt Birgit EU Marie Curie Chair, ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, IIFA-Instituto de Formação e Investigação Avançada, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554, Évora, Portugal aut Planta Springer Berlin Heidelberg 241/2(2015-02-01), 525-538 0032-0935 241:2<525 2015 241 425 https://doi.org/10.1007/s00425-014-2195-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2195-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nogales Amaia EU Marie Curie Chair, ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, IIFA-Instituto de Formação e Investigação Avançada, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554, Évora, Portugal aut NATIONALLICENCE 700 1- Muñoz-Sanhueza Luz Department of Plant Sciences (IPV), Norwegian University of Life Sciences (NMBU), P.O. Box 5003, 1432, Ås, Norway aut NATIONALLICENCE 700 1- Hansen Lee Department of Chemistry and Biochemistry, Brigham Young University, 84602, Provo, UT, USA aut NATIONALLICENCE 700 1- Arnholdt-Schmitt Birgit EU Marie Curie Chair, ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, IIFA-Instituto de Formação e Investigação Avançada, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554, Évora, Portugal aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/2(2015-02-01), 525-538 0032-0935 241:2<525 2015 241 425