caa a22 4500 606209530 CHVBK 20210128101029.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00425-014-2200-5 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2200-5 Soybean chalcone isomerase: evolution of the fold, and the differential expression and localization of the gene family [Elektronische Daten] [Mehran Dastmalchi, Sangeeta Dhaubhadel] Main conclusion : Soybean chalcone isomerase (CHI) family contains twelve members with unique evolutionary background, expression patterns and is compartmentalized to specific subcellular locations. The phenylpropanoid pathway produces a diverse array of plant natural products. A key branch-point enzyme, chalcone isomerase, catalyzes the reaction producing flavanones, the backbone for many downstream metabolites such as flavonoids and isoflavonoids. We have identified twelve soybean GmCHIs that fall into four subfamilies. The study of this family in soybean in the context of various CHIs and CHI-like proteins, across divisions in the plant kingdom and beyond, shows an evolutionary journey from fatty acid-binding proteins (FAPs) to sterically restricted folds that gave rise to the chalcone-to-flavanone isomerase. There are four GmCHIs with this functionality, three of which belong to a legume-specific clade known as ‘type II' CHIs. Tissue-specific expression of eight core members of the soybean CHI family showed differential temporal and spatial expression, pointing to the potential function of GmCHI1A in seed isoflavonoid production. Promoter analysis of the GmCHIs described the minutiae of sub-organ expression patterns. Subcellular localization of the family was conducted to investigate the possibility of pathway-specific compartmentalization. Subfamilies 1, 2 and 4 localized to the nucleus and cytoplasm, with nuclear localization of CHIs raising questions about alternate function. GmCHI3 isoforms localized to the chloroplast, which, in conjunction with their position on the phylogenetic tree and expression patterns, closely associates them with the FAPs. This study provides the first comprehensive look at soybean CHIs, a family of unique evolutionary background and biochemical function, with the catalytically active members producing the backbone substrate in an important plant metabolic pathway. Her Majesty the Queen in Right of Canada, 2014 Phenylpropanoid nationallicence Isoflavonoids nationallicence Soybean nationallicence Chalcone isomerase nationallicence Fatty acid-binding proteins nationallicence Dastmalchi Mehran Department of Biology, University of Western Ontario, London, ON, Canada aut Dhaubhadel Sangeeta Department of Biology, University of Western Ontario, London, ON, Canada aut Planta Springer Berlin Heidelberg 241/2(2015-02-01), 507-523 0032-0935 241:2<507 2015 241 425 https://doi.org/10.1007/s00425-014-2200-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2200-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dastmalchi Mehran Department of Biology, University of Western Ontario, London, ON, Canada aut NATIONALLICENCE 700 1- Dhaubhadel Sangeeta Department of Biology, University of Western Ontario, London, ON, Canada aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/2(2015-02-01), 507-523 0032-0935 241:2<507 2015 241 425