caa a22 4500 606209549 CHVBK 20210128101029.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00425-014-2194-z doi (NATIONALLICENCE)springer-10.1007/s00425-014-2194-z The life of phi: the development of phi thickenings in roots of the orchids of the genus Miltoniopsis [Elektronische Daten] [Nurul Idris, David Collings] Phi thickenings, bands of secondary wall thickenings that reinforce the primary wall of root cortical cells in a wide range of species, are described for the first time in the epiphytic orchid Miltoniopsis. As with phi thickenings found in other plants, the phi thickenings in Miltoniopsis contain highly aligned cellulose running along the lengths of the thickenings, and are lignified but not suberized. Using a combination of histological and immunocytochemical techniques, thickening development can be categorized into three different stages. Microtubules align lengthwise along the thickening during early and intermediate stages of development, and callose is deposited within the thickening in a pattern similar to the microtubules. These developing thickenings also label with the fluorescently tagged lectin wheat germ agglutinin (WGA). These associations with microtubules and callose, and the WGA labeling, all disappear when the phi thickenings are mature. This pattern of callose and WGA deposition show changes in the thickened cell wall composition and may shed light on the function of phi thickenings in plant roots, a role for which has yet to be established. Springer-Verlag Berlin Heidelberg, 2014 Callose nationallicence Cellulose deposition nationallicence Orchids nationallicence Microtubule organization nationallicence Miltoniopsis nationallicence Mycorrhizae nationallicence Phi thickenings nationallicence Wheat germ agglutinin nationallicence WGA : Wheat germ agglutinin nationallicence Idris Nurul School of Biological Science, University of Canterbury, Private Bag 4800, 8140, Christchurch, New Zealand aut Collings David School of Biological Science, University of Canterbury, Private Bag 4800, 8140, Christchurch, New Zealand aut Planta Springer Berlin Heidelberg 241/2(2015-02-01), 489-506 0032-0935 241:2<489 2015 241 425 https://doi.org/10.1007/s00425-014-2194-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2194-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Idris Nurul School of Biological Science, University of Canterbury, Private Bag 4800, 8140, Christchurch, New Zealand aut NATIONALLICENCE 700 1- Collings David School of Biological Science, University of Canterbury, Private Bag 4800, 8140, Christchurch, New Zealand aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/2(2015-02-01), 489-506 0032-0935 241:2<489 2015 241 425