caa a22 4500 606209557 CHVBK 20210128101029.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00425-014-2176-1 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2176-1 Fungal hemicellulose-degrading enzymes cause physical property changes concomitant with solubilization of cell wall polysaccharides [Elektronische Daten] [Machiko Takahashi, Ryoichi Yamamoto, Naoki Sakurai, Yuki Nakano, Takumi Takeda] Main conclusion : Physical properties of wheat coleoptile segments decreased after treatment with hemicellulose-degrading enzymes, indicating that hemicellulosic polysaccharides function to control the strength of primary cell walls. Abstract: Changes in the physical properties of plant cell walls, a viscoelastic structure, are thought to be one of the growth-limiting factors for plants and one of the infection-affecting factors for fungi. To study the significance of hemicellulosic polysaccharides that form cross-bridges between cellulose microfibrils in controlling cell wall strength in monocot plants, the effects of hemicellulose degradation by recombinant Magnaporthe oryzae xylanase and 1,3-1,4-β-glucanase, and recombinant Aspergillus oryzae xyloglucanase on the physical properties and polysaccharide solubilization were investigated using wheat (Triticum aestivum L.) coleoptiles. Treatments with xylanase or 1,3-1,4-β-glucanase significantly decreased the viscosity and elasticity of wheat coleoptile segments. In addition, xyloglucanase treatment slightly decreased the viscoelasticity. Furthermore, 1,3-1,4-β-glucan polymer was solubilized during hydrolysis with xylanase and xyloglucanase, even though neither enzyme had hydrolytic activity towards 1,3-1,4-β-glucan. These results suggest that xylan and xyloglucan interact with 1,3-1,4-β-glucan and that the composites and hemicellulosic polysaccharides form inter-molecular bridges. Degradation of these bridges causes decreases in the physical properties, resulting in increased extensibility of the cell walls. These findings provide a testable model in which wheat coleoptile cell walls are loosened by the degradation of hemicellulosic polysaccharides and hemicellulose-degrading enzymes play a significant role in loosening the walls during fungal infection. Springer-Verlag Berlin Heidelberg, 2014 Xylanase nationallicence 1,3-1,4-β-Glucanase nationallicence Viscoelastic properties nationallicence Hemicellulosic tethers nationallicence Polysaccharide solubilization nationallicence Takahashi Machiko Iwate Biotechnology Research Center, 22-174-4 Narita, 024-0003, Kitakami, Iwate, Japan aut Yamamoto Ryoichi Institute of Human and Environmental Sciences, Tezukayama University, 3-1-1 Gakuen-minami, 631-8585, Nara, Japan aut Sakurai Naoki Graduate School of Biosphere Science, Hiroshima University, 1-7-1 Kagamiyama, 739-8528, Higashihiroshima, Japan aut Nakano Yuki Iwate Biotechnology Research Center, 22-174-4 Narita, 024-0003, Kitakami, Iwate, Japan aut Takeda Takumi Iwate Biotechnology Research Center, 22-174-4 Narita, 024-0003, Kitakami, Iwate, Japan aut Planta Springer Berlin Heidelberg 241/2(2015-02-01), 359-370 0032-0935 241:2<359 2015 241 425 https://doi.org/10.1007/s00425-014-2176-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2176-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi Machiko Iwate Biotechnology Research Center, 22-174-4 Narita, 024-0003, Kitakami, Iwate, Japan aut NATIONALLICENCE 700 1- Yamamoto Ryoichi Institute of Human and Environmental Sciences, Tezukayama University, 3-1-1 Gakuen-minami, 631-8585, Nara, Japan aut NATIONALLICENCE 700 1- Sakurai Naoki Graduate School of Biosphere Science, Hiroshima University, 1-7-1 Kagamiyama, 739-8528, Higashihiroshima, Japan aut NATIONALLICENCE 700 1- Nakano Yuki Iwate Biotechnology Research Center, 22-174-4 Narita, 024-0003, Kitakami, Iwate, Japan aut NATIONALLICENCE 700 1- Takeda Takumi Iwate Biotechnology Research Center, 22-174-4 Narita, 024-0003, Kitakami, Iwate, Japan aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/2(2015-02-01), 359-370 0032-0935 241:2<359 2015 241 425