caa a22 4500 606209581 CHVBK 20210128101029.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00425-014-2193-0 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2193-0 miRNA expression during prickly pear cactus fruit development [Elektronische Daten] [Flor Rosas-Cárdenas, Juan Caballero-Pérez, Ximena Gutiérrez-Ramos, Nayelli Marsch-Martínez, Andrés Cruz-Hernández, Stefan de Folter] miRNAs are a class of small non-coding RNAs that regulate gene expression. They are involved in the control of many developmental processes, including fruit development. The increasing amount of information on miRNAs, on their expression, abundance, and conservation between various species, provides a new opportunity to study the role of miRNAs in non-model plant species. In this work, we used a combination of Northern blot and tissue print hybridization analysis to identify conserved miRNAs expressed during prickly pear cactus (Opuntia ficus indica) fruit development. Comparative profiling detected the expression of 34 miRNAs, which were clustered in three different groups that were associated with the different phases of fruit development. Variation in the level of miRNA expression was observed. Gradual expression increase of several miRNAs was observed during fruit development, including miR164. miR164 was selected for stem-loop RT-PCR and for a detailed spatial-temporal expression analysis. At early floral stages, miR164 was mainly localized in meristematic tissues, boundaries and fusion zones, while it was more homogenously expressed in fruit tissues. Our results provide the first evidence of miRNA expression in the prickly pear cactus and provide the basis for future research on miRNAs in Opuntia. Moreover, our analyses suggest that miR164 plays different roles during prickly pear cactus fruit development. Springer-Verlag Berlin Heidelberg, 2014 Fruit nationallicence miRNAs nationallicence miR164 nationallicence Opuntia nationallicence Prickly pear cactus nationallicence sRNA : Small RNA nationallicence miRNA : microRNA nationallicence LMW : Low molecular weight nationallicence HMW : High molecular weight nationallicence EDC : 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide nationallicence Rosas-Cárdenas Flor Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Km. 9.6 Libramiento Norte, Carretera Irapuato-León, CP 36821, Irapuato, Guanajuato, Mexico aut Caballero-Pérez Juan Unidad de Microbiología, Facultad de Ingeniería, Campus Aeropuerto, Universidad Autónoma de Querétaro, CP 76140, Santiago De Querétaro, Querétaro, Mexico aut Gutiérrez-Ramos Ximena Unidad de Microbiología, Facultad de Ingeniería, Campus Aeropuerto, Universidad Autónoma de Querétaro, CP 76140, Santiago De Querétaro, Querétaro, Mexico aut Marsch-Martínez Nayelli Departamento de Biotecnología y Bioquímica, CINVESTAV-IPN, Km. 9.6 Libramiento Norte, Carretera Irapuato-León, CP 36821, Irapuato, Guanajuato, Mexico aut Cruz-Hernández Andrés Unidad de Microbiología, Facultad de Ingeniería, Campus Aeropuerto, Universidad Autónoma de Querétaro, CP 76140, Santiago De Querétaro, Querétaro, Mexico aut de Folter Stefan Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Km. 9.6 Libramiento Norte, Carretera Irapuato-León, CP 36821, Irapuato, Guanajuato, Mexico aut Planta Springer Berlin Heidelberg 241/2(2015-02-01), 435-448 0032-0935 241:2<435 2015 241 425 https://doi.org/10.1007/s00425-014-2193-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2193-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rosas-Cárdenas Flor Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Km. 9.6 Libramiento Norte, Carretera Irapuato-León, CP 36821, Irapuato, Guanajuato, Mexico aut NATIONALLICENCE 700 1- Caballero-Pérez Juan Unidad de Microbiología, Facultad de Ingeniería, Campus Aeropuerto, Universidad Autónoma de Querétaro, CP 76140, Santiago De Querétaro, Querétaro, Mexico aut NATIONALLICENCE 700 1- Gutiérrez-Ramos Ximena Unidad de Microbiología, Facultad de Ingeniería, Campus Aeropuerto, Universidad Autónoma de Querétaro, CP 76140, Santiago De Querétaro, Querétaro, Mexico aut NATIONALLICENCE 700 1- Marsch-Martínez Nayelli Departamento de Biotecnología y Bioquímica, CINVESTAV-IPN, Km. 9.6 Libramiento Norte, Carretera Irapuato-León, CP 36821, Irapuato, Guanajuato, Mexico aut NATIONALLICENCE 700 1- Cruz-Hernández Andrés Unidad de Microbiología, Facultad de Ingeniería, Campus Aeropuerto, Universidad Autónoma de Querétaro, CP 76140, Santiago De Querétaro, Querétaro, Mexico aut NATIONALLICENCE 700 1- de Folter Stefan Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Km. 9.6 Libramiento Norte, Carretera Irapuato-León, CP 36821, Irapuato, Guanajuato, Mexico aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/2(2015-02-01), 435-448 0032-0935 241:2<435 2015 241 425