caa a22 4500 606209603 CHVBK 20210128101029.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00425-014-2191-2 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2191-2 Biological activities of the antiviral protein BE27 from sugar beet ( Beta vulgaris L.) [Elektronische Daten] [Rosario Iglesias, Lucía Citores, Antimo Di Maro, José Ferreras] Main conclusion : The ribosome inactivating protein BE27 displays several biological activities in vitro that could result in a broad action against several types of pathogens. Beetin 27 (BE27), a ribosome-inactivating protein (RIP) from sugar beet (Beta vulgaris L.) leaves, is an antiviral protein induced by virus and signaling compounds such as hydrogen peroxide and salicylic acid. Its role as a defense protein has been attributed to its RNA polynucleotide:adenosine glycosidase activity. Here we tested other putative activities of BE27 that could have a defensive role against pathogens finding that BE27 displays rRNA N-glycosidase activity against yeast and Agrobacterium tumefaciens ribosomes, DNA polynucleotide:adenosine glycosidase activity against herring sperm DNA, and magnesium-dependent endonuclease activity against the supercoiled plasmid PUC19 (nicking activity). The nicking activity could be a consequence of an unusual conformation of the BE27 active site, similar to that of PD-L1, a RIP from Phytolacca dioica L. leaves. Additionally, BE27 possesses superoxide dismutase activity, thus being able to produce the signal compound hydrogen peroxide. BE27 is also toxic to COLO 320 cells, inducing apoptosis in these cells by either activating the caspase pathways and/or inhibiting protein synthesis. The combined effect of these biological activities could result in a broad action against several types of pathogens such as virus, bacteria, fungi or insects. Springer-Verlag Berlin Heidelberg, 2014 Antifungal nationallicence Apoptosis nationallicence Caspase nationallicence Endonuclease nationallicence N -glycosidase nationallicence Superoxide dismutase nationallicence NBT : 4-Nitro blue tetrazolium nationallicence SOD : Superoxide dismutase nationallicence SRL : Sarcin ricin loop nationallicence Iglesias Rosario Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, 47011, Valladolid, Spain aut Citores Lucía Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, 47011, Valladolid, Spain aut Di Maro Antimo Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, 81100, Caserta, Italy aut Ferreras José Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, 47011, Valladolid, Spain aut Planta Springer Berlin Heidelberg 241/2(2015-02-01), 421-433 0032-0935 241:2<421 2015 241 425 https://doi.org/10.1007/s00425-014-2191-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2191-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iglesias Rosario Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- Citores Lucía Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- Di Maro Antimo Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, 81100, Caserta, Italy aut NATIONALLICENCE 700 1- Ferreras José Department of Biochemistry and Molecular Biology and Physiology, Faculty of Sciences, University of Valladolid, 47011, Valladolid, Spain aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/2(2015-02-01), 421-433 0032-0935 241:2<421 2015 241 425