caa a22 4500 60620962X CHVBK 20210128101029.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00425-014-2177-0 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2177-0 Structural features of diverse Pin-II proteinase inhibitor genes from Capsicum annuum [Elektronische Daten] [Neha Mahajan, Veena Dewangan, Purushottam Lomate, Rakesh Joshi, Manasi Mishra, Vidya Gupta, Ashok Giri] Main conclusion : The proteinase inhibitor (PI) genes from Capsicum annuum were characterized with respect to their UTR, introns and promoter elements. The occurrence of PIs with circularly permuted domain organization was evident. Abstract: Several potato inhibitor II (Pin-II) type proteinase inhibitor (PI) genes have been analyzed from Capsicum annuum (L.) with respect to their differential expression during plant defense response. However, complete gene characterization of any of these C. annuum PIs (CanPIs) has not been carried out so far. Complete gene architectures of a previously identified CanPI-7 (Beads-on-string, Type A) and a member of newly isolated Bracelet type B, CanPI-69 are reported in this study. The 5′ UTR (untranslated region), 3′UTR, and intronic sequences of both the CanPI genes were obtained. The genomic sequence of CanPI-7 exhibited, exon 1 (49 base pair, bp) and exon 2 (740bp) interrupted by a 294-bp long type I intron. We noted the occurrence of three multi-domain PIs (CanPI-69, 70, 71) with circularly permuted domain organization. CanPI-69 was found to possess exon 1 (49bp), exon 2 (551bp) and a 584-bp long type I intron. The upstream sequence analysis of CanPI-7 and CanPI-69 predicted various transcription factor-binding sites including TATA and CAAT boxes, hormone-responsive elements (ABRELATERD1, DOFCOREZM, ERELEE4), and a defense-responsive element (WRKY71OS). Binding of transcription factors such as zinc finger motif MADS-box and MYB to the promoter regions was confirmed using electrophoretic mobility shift assay followed by mass spectrometric identification. The 3′ UTR analysis for 25 CanPI genes revealed unique/distinct 3′ UTR sequence for each gene. Structures of three domain CanPIs of type A and B were predicted and further analyzed for their attributes. This investigation of CanPI gene architecture will enable the better understanding of the genetic elements present in CanPIs. Springer-Verlag Berlin Heidelberg, 2014 Capsicum nationallicence CanPI nationallicence Gene architecture nationallicence Promoter nationallicence Proteinase inhibitor nationallicence CanPI : Capsicum annuum proteinase inhibitor nationallicence UTR : Untranslated region nationallicence NPE : Nuclear protein extract nationallicence EMSA : Electrophoretic mobility shift assay nationallicence MS : Mass spectrometry nationallicence Mahajan Neha Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Dewangan Veena Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Lomate Purushottam Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Joshi Rakesh Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Mishra Manasi Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Gupta Vidya Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Giri Ashok Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Planta Springer Berlin Heidelberg 241/2(2015-02-01), 319-331 0032-0935 241:2<319 2015 241 425 https://doi.org/10.1007/s00425-014-2177-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2177-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mahajan Neha Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Dewangan Veena Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Lomate Purushottam Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Joshi Rakesh Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Mishra Manasi Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Gupta Vidya Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Giri Ashok Division of Biochemical Sciences, Plant Molecular Biology Unit, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/2(2015-02-01), 319-331 0032-0935 241:2<319 2015 241 425