caa a22 4500 606209646 CHVBK 20210128101029.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00425-014-2190-3 doi (NATIONALLICENCE)springer-10.1007/s00425-014-2190-3 Distinct subfunctionalization and neofunctionalization of the B-class MADS-box genes in Physalis floridana [Elektronische Daten] [Shaohua Zhang, Ji-Si Zhang, Jing Zhao, Chaoying He] Main conclusion : This work suggested that in Physalis PFGLO1-PFDEF primarily determined corolla and androecium identity, and acquired a novel role in gynoecia functionality, while PFGLO2-PFTM6 functioned in pollen maturation only. Abstract: The B-class MADS-box genes play a crucial role in determining the organ identity of the corolla and androecium. Two GLOBOSA-like (GLO-like) PFGLO1 and PFGLO2 and two DEFICIENS-like (DEF-like) PFDEF and PFTM6 genes were present in Physalis floridana. However, the double-layered-lantern1 (doll1) mutant is the result of a single recessive mutation in PFGLO1, hinting a distinct divergent pattern of B-class genes. In this work, we utilized the tobacco rattle virus (TRV)-mediated gene silencing approach to further verify this assumption in P. floridana. Silencing of PFGLO1 or/and PFDEF demonstrated their primary role in determining corolla and androecium identity. However, specific PFGLO2 or/and PFTM6 silencing did not affect any organ identity but showed a reduction in mature pollen. These results suggested that both PFGLO2 and PFTM6 had lost their role in organ identity determination but functioned in pollen maturation. Evaluation of fruit setting in reciprocal crosses suggested that both PFGLO1 and PFDEF might have acquired an essential and novel role in the functionality of gynoecia. Such a divergence of the duplicated GLO-DEF heterodimer genes in floral development is different from the existing observations within Solanaceae. Therefore, our research sheds new light on the functional evolution of the duplicated B-class MADS-box genes in angiosperms. Springer-Verlag Berlin Heidelberg, 2014 MADS-box gene nationallicence Functional evolution nationallicence Fertility nationallicence Organ identity nationallicence Physalis nationallicence BiFC : Bimolecular fluorescence complementation nationallicence cDNA : Complementary DNA nationallicence GFP : Green fluorescence protein nationallicence ORF : Open reading frame nationallicence TRV : Tobacco rattle virus nationallicence VIGS : Virus-induced gene silencing nationallicence YFP : Yellow fluorescence protein nationallicence Zhang Shaohua State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, 100093, Beijing, China aut Zhang Ji-Si State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, 100093, Beijing, China aut Zhao Jing State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, 100093, Beijing, China aut He Chaoying State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, 100093, Beijing, China aut Planta Springer Berlin Heidelberg 241/2(2015-02-01), 387-402 0032-0935 241:2<387 2015 241 425 https://doi.org/10.1007/s00425-014-2190-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2190-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Shaohua State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, 100093, Beijing, China aut NATIONALLICENCE 700 1- Zhang Ji-Si State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, 100093, Beijing, China aut NATIONALLICENCE 700 1- Zhao Jing State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, 100093, Beijing, China aut NATIONALLICENCE 700 1- He Chaoying State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, 100093, Beijing, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/2(2015-02-01), 387-402 0032-0935 241:2<387 2015 241 425