caa a22 4500 606209670 CHVBK 20210128101029.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00425-014-2187-y doi (NATIONALLICENCE)springer-10.1007/s00425-014-2187-y Identification of differentially expressed transcripts associated with bast fibre development in Corchorus capsularis by suppression subtractive hybridization [Elektronische Daten] [Pradipta Samanta, Sanjoy Sadhukhan, Asitava Basu] Main conclusion : The present study documented the predominant role of WRKY transcription factor in controlling genes of different pathways related to fibre formation in jute and could be a candidate gene for the improvement of jute fiber. Abstract: Understanding of molecular mechanism associated with bast fibre development is of immense significance to achieve desired improvement in jute (Corchorus sp.). Therefore, suppression subtractive hybridization was successfully applied to identify genes involved in fibre developmental process in jute. The subtracted library of normal Corchorus capsularis as tester with respect to its fibre-deficient mutant as driver resulted in 2,685 expressed sequence tags which were assumed to represent the differentially expressed genes between two genotypes. The identified expressed sequence tags were assembled and clustered into 225 contigs and 231 singletons. Among these 456 unigenes, 377 were classified into 15 different functional categories while others were of unknown functional category. Reverse Northern analysis of the unigenes showed distinct variation in hybridization intensity of 11 transcripts between two genotypes tested. The findings were also documented by Northern and real-time PCR analysis. Varied expression level of these transcripts suggested their crucial involvement in fibre development in this species. Among these transcripts, WRKY transcription factor was documented to be a most important transcript which was in agreement with its known role in other plant species in possible regulation related to cell wall biosynthesis, expansion and lignification. This report constitutes first systematic analysis of genes involved in fibre development process in jute. It may be suggested that the information generated in this study would be useful for genetic improvement of fibre traits in this plant species. Springer-Verlag Berlin Heidelberg, 2014 Corchorus nationallicence Expressed sequence tags nationallicence Fibre-deficient mutant nationallicence Northern hybridization nationallicence Real-time PCR nationallicence Suppression subtractive hybridization nationallicence AGP : Arabinogalactan protein nationallicence CAD : Cinnamyl alcohol dehydrogenase nationallicence CCR : Cinnamoyl-CoA reductase nationallicence C4H : Cinnamate-4-hydroxylase nationallicence CHS : Chalcone synthase nationallicence 4CL : 4-Coumarate CoA:ligase nationallicence COMT : Caffeic acid 3-O-methyltransferase nationallicence Das : Days after sowing nationallicence EST : Expressed sequence tags nationallicence FF : Fibre forming nationallicence F5H : Ferulic acid/coniferaldehyde/coniferyl alcohol 5-hydoxylase nationallicence GRP : Glycine rich protein nationallicence HCT : Hydroxycinnamoyl CoA:shikimate hydroxycinnamoyl transferase nationallicence HD-Zip : Homeodomain leucine zipper protein nationallicence PAL : Phenylalanine ammonia lyase nationallicence PEL : Pectate lyase nationallicence PME : Pectin methylesterase nationallicence SSH : Suppression subtractive hybridization nationallicence XET : Xyloglucan endotransglycosylase nationallicence ZFP : Zinc finger protein nationallicence Samanta Pradipta Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Sadhukhan Sanjoy Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Basu Asitava Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut Planta Springer Berlin Heidelberg 241/2(2015-02-01), 371-385 0032-0935 241:2<371 2015 241 425 https://doi.org/10.1007/s00425-014-2187-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-014-2187-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Samanta Pradipta Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 700 1- Sadhukhan Sanjoy Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 700 1- Basu Asitava Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, 721302, Kharagpur, India aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 241/2(2015-02-01), 371-385 0032-0935 241:2<371 2015 241 425